Molecular Characteristics of the Serological Weak D Phenotype in Koreans

Diagnostics

KyoungUn Park¹  YunJi Hong¹  Dajeong Jeong¹  EunYoung Song¹  Sujin Oh¹ 

[1] Department of Laboratory Medicine, Seoul National University College of Medicine, Seoul 03080, Korea;

关键词: weak D;    partial D;    RhD variant;    PCR-SSP;    real-time PCR;   

DOI  :  10.3390/diagnostics11060920

来源: DOAJ

【 摘 要 】

Serological weak D is a reaction of 2+ or less to anti-D reagent and includes weak D and partial D phenotypes. Although identifying the RhD subtype is important for transfusion safety, serological tests are insufficient for defining the RhD subtype, and molecular tests are needed. To analyze the molecular characteristics of D variants in Koreans to facilitate the formulation of individualized transfusion strategies, molecular tests such as RhD genotyping using real-time polymerase chain reaction (PCR) and partial-D and/or weak-D sequence-specific amplification (SSP) were performed on 105 Korean Rare Blood Program (KRBP) patients exhibiting serological weak D. In total, 58 out of 68 serologically determined weak D KRBP patients were typed as having weak D or partial D phenotypes via RhD genotyping. In detail, eight (13.8%) were typed as partial DVa or DBS, nine (15.5%) as weak D type 15, and four others (6.8%) as partial DVI, partial DVII, weak D type 2, or weak D type 41 or 45, whereas the rest (n = 37, 63.8%) was typed as having either weak D or partial D. This suggests that serological weak D Koreans who require transfusion should be treated as D-negative.

【 授权许可】

Unknown