Optimization of Sample Preparation for the Identification and Quantification of Saxitoxin in Proficiency Test Mussel Sample using Liquid Chromatography-Tandem Mass Spectrometry

Toxins

Werner Luginbühl¹  Martin Schär²  Werner Arnold²  Marc-André Avondet²  Stephen Burrell³  Kirsi Harju⁴  Marja-Leena Rapinoja⁴  Paula Vanninen⁴ 

[1] ChemStat, Aarstrasse 98, Bern CH-3005, Switzerland;Federal Department of Defence, Civil Protection and Sport, SPIEZ LABORATORY, Austrasse 1, Spiez CH-3700, Switzerland;Marine Institute, Marine Environment and Food Safety Services, Rinville, Oranmore, Co. Galway, Ireland;VERIFIN (Finnish Institute for Verification of the Chemical Weapons Convention), Department of Chemistry, University of Helsinki, P.O. Box 55, A. I. Virtasen aukio 1 FI-00014, Finland;

关键词: paralytic shellfish poisoning toxins;    saxitoxin;    liquid chromatography-mass spectrometry;    mussel;   

DOI  :  10.3390/toxins7124853

来源: DOAJ

【 摘 要 】

Saxitoxin (STX) and some selected paralytic shellfish poisoning (PSP) analogues in mussel samples were identified and quantified with liquid chromatography-tandem mass spectrometry (LC-MS/MS). Sample extraction and purification methods of mussel sample were optimized for LC-MS/MS analysis. The developed method was applied to the analysis of the homogenized mussel samples in the proficiency test (PT) within the EQuATox project (Establishment of Quality Assurance for the Detection of Biological Toxins of Potential Bioterrorism Risk). Ten laboratories from eight countries participated in the STX PT. Identification of PSP toxins in naturally contaminated mussel samples was performed by comparison of product ion spectra and retention times with those of reference standards. The quantitative results were obtained with LC-MS/MS by spiking reference standards in toxic mussel extracts. The results were within the z-score of ±1 when compared to the results measured with the official AOAC (Association of Official Analytical Chemists) method 2005.06, pre-column oxidation high-performance liquid chromatography with fluorescence detection (HPLC-FLD).

【 授权许可】

Unknown