Progress in Allergy Signal Research on Mast Cells: The Role of Histamine in Goblet Cell Hyperplasia in Allergic Airway Inflammation –a Study Using the Hdc Knockout Mouse

Journal of Pharmacological Sciences

Hong Mei Piao¹  Toshiki Shikanai²  Hiroshi Inoue³  Hiroshi Ohtsu⁴  Chihaya Maesawa⁵  Hitoshi Kobayashi⁵  Yutaka Nakamura⁵  Miyuki Niisato⁵  Toshihide Nakadate⁵  Kohei Yamauchi⁵  Takashi Mouri⁵  Harumasa Ito⁵  Takashi Sawai⁵ 

[1] Corresponding author. [e-mail];Department of Respiratory Medicine, Affiliated Hospital of Yanbian University College of Medicine, Yanji, China;First Department of Pathology, Iwate Medical University School of Medicine, 19-1 Uchimaru, Morioka 020-8585, Japan;Second Department of Pathology, 19-1 Uchimaru, Morioka 020-8585, Japan;Third Department of Internal Medicine, 19-1 Uchimaru, Morioka 020-8585, Japan;

DOI  :  

来源: DOAJ

【 摘 要 】

Although histamine is a central mediator in the immediate allergic reaction, its role in goblet cell hyperplasia in the airway of asthma is not completely understood. This study was designed to examine the role of histamine in goblet cell hyperplasia using histamine-deficient mice (Hdc −/−mice) with allergic airway inflammation. Wild-type and Hdc −/−C57BL/6 mice were sensitized with ovalbumin (OVA). After two-week exposure to OVA, goblet cell hyperplasia was evaluated. Cell differentials in BALF were analyzed. The mRNAs level of MUC5AC and Gob-5 gene were quantitatively determined. The number of eosinophils in BALF increased in both the wild-type mice and Hdc −/−mice; however, their ratio in Hdc −/−mice was significantly lower than that in the wild-type mice. The mRNA levels of Gob-5 and MUC5AC and the ratio of the goblet cells in the airway epithelium were significantly increased in Hdc −/−mice exposed to OVA compared to the wild-type mice under the same condition. These results suggested that histamine may play a regulatory role in goblet cell hyperplasia in allergic airway inflammation. Keywords:: airway remodeling, asthma, Gob-5, MUC5AC, allergy

【 授权许可】

Unknown