Differential chemical imaging of extracellular acidification within microfluidic channels using a plasma-functionalized light-addressable potentiometric sensor (LAPS)

Physics in Medicine

Sefa Kizildag¹  Dua Özsoylu²  Torsten Wagner²  Michael J. Schöning³ 

[1] Medical Biology and Genetics, Graduate School of Health Sciences, Dokuz Eylül University, Balcova, 35340, Izmir, Turkey;Institute of Nano- and Biotechnologies (INB), Aachen University of Applied Sciences, Campus Jülich, 52428, Jülich, Germany;Medical Biology and Genetics, Graduate School of Health Sciences, Dokuz Eylül University, Balcova, 35340, Izmir, Turkey;

关键词: Light-addressable potentiometric;    Sensor;    Plasma treatment;    Differential chemical image;    Extracellular acidification;    Microfluidic chip system;   

DOI  :  

来源: DOAJ

【 摘 要 】

Extracellular acidification is a basic indicator for alterations in two vital metabolic pathways: glycolysis and cellular respiration. Measuring these alterations by monitoring extracellular acidification using cell-based biosensors such as LAPS plays an important role in studying these pathways whose disorders are associated with numerous diseases including cancer. However, the surface of the biosensors must be specially tailored to ensure high cell compatibility so that cells can represent more in vivo-like behavior, which is critical to gain more realistic in vitro results from the analyses, e.g., drug discovery experiments. In this work, O2 plasma patterning on the LAPS surface is studied to enhance surface features of the sensor chip, e.g., wettability and biofunctionality. The surface treated with O2 plasma for 30 s exhibits enhanced cytocompatibility for adherent CHO–K1 cells, which promotes cell spreading and proliferation. The plasma-modified LAPS chip is then integrated into a microfluidic system, which provides two identical channels to facilitate differential measurements of the extracellular acidification of CHO–K1 cells. To the best of our knowledge, it is the first time that extracellular acidification within microfluidic channels is quantitatively visualized as differential (bio-)chemical images.

【 授权许可】

Unknown