Insights into the role of reactive sulfhydryl groups of Carbonic Anhydrase III and VII during oxidative damage

Journal of Enzyme Inhibition and Medicinal Chemistry

Giuseppina De Simone¹  Simona M. Monti¹  Emma Langella¹  Anna Di Fiore¹  Daria M. Monti²  Claudiu T. Supuran³ 

[1] Institute of Biostructures and Bioimaging, CNR;University of Naples Federico II;Università degli Studi di Firenze;

关键词: Carbonic anhydrase III;    carbonic anhydrase VII;    S-glutathiolation;    oxidative stress;    reactive cysteines;    sulfhydryl groups;   

DOI  :  10.1080/14756366.2016.1225046

来源: DOAJ

【 摘 要 】

Carbonic anhydrases (CAs) III and VII are two cytosolic isoforms of the α-CA family which catalyze the physiological reaction of carbon dioxide hydration to bicarbonate and proton. Despite these two enzymes share a 49% sequence identity and present a very similar three-dimensional structure, they show profound differences when comparing the specific activity for CO2 hydration reaction, with CA VII being much more active than CA III. Recently, CA III and CA VII have been proposed to play a new role as scavenger enzymes in cells where oxidative damage occurs. Here, we will examine functional and structural features of these two isoforms giving insights into their newly proposed protective role against oxidative stress.

【 授权许可】

Unknown