Combined Immunoscore for Prognostic Stratification of Early Stage Non-Small-Cell Lung Cancer

Frontiers in Oncology

Alice Boscolo¹  Giulia Pasello²  PierFranco Conte²  Valentina Guarneri²  Jessica Menis²  Stefano Frega²  Loredana Urso²  Laura Bonanno²  Francesco Fortarezza³  Fiorella Calabrese³  Francesca Lunardi³  Giovanni Comacchio⁴  Federico Rea⁴ 

[1] Department of Surgery, Oncology and Gastroenterology, University of Padua, Padua, Italy;Oncology Unit 2, Istituto Oncologico Veneto IRCCS, Padua, Italy;Pathology Unit, Department of Cardiac, Thoracic, Vascular Sciences and Public Health, University of Padua, Padua, Italy;Thoracic Surgery Unit, Department of Cardiac, Thoracic, Vascular Sciences and Public Health, University of Padua, Padua, Italy;

关键词: NSCLC;    immune microenvironment;    early stage;    prognosis;    PD-L1;   

DOI  :  10.3389/fonc.2020.564915

来源: DOAJ

【 摘 要 】

BackgroundTo date, no combined immunoscore has been evaluated for prognostic stratification of early stage non-small-cell lung cancer (NSCLC). The main goal of this study was to investigate the prognostic impact of programmed death ligand 1 (PD-L1) expression and different immune cell components (CD4+, CD8+ T-lymphocytes, and CD68+ macrophages) in early stage NSCLC patients, distinguishing peritumoral (PT) and intratumoral (IT) localizations. The secondary aim was to identify a combined immunoscore to optimize the prognostic stratification of NSCLC patients.MethodsThis retrospective study included surgical specimens from consecutive chemo-naive stage II–III radically resected NSCLC patients. Immunohistochemistry was carried out to evaluate PD-L1 expression and to quantify IT and PT CD4+, CD8+ T-lymphocytes, and CD68+ macrophages. The impact of a single marker and of a combination of multiple markers on overall survival (OS) was investigated.ResultsSeventy-nine patients were included in the study. PD-L1 expression was associated with worse prognosis (3 years OS: 58% in high- compared with 67% in low-expressing tumors), even though without statistical significance. When integrating PT CD8+, CD4+, and CD68 into a combined PT immunoscore, a significant prognostic stratification of patients was obtained and confirmed at multivariate analysis (3 years OS: 86% in patients with low PT immunoscore vs. 59% in patients with high PT immunoscore, p = 0.018). The integration of derived neutrophil/lymphocyte ratio (dNLR) with combined PT immunoscore improved prognostic stratification, with longer OS in patients with low PT immunoscore and low dNLR (p = 0.002).ConclusionThe combined PT immunoscore (CD8+, CD4+, and CD68) integrated with dNLR may be a promising marker for the development of an integrated Tumor, Node, Metastasis (TNM) immunoscore.

【 授权许可】

Unknown