Experimental and Model-Based Analysis to Optimize Microalgal Biomass Productivity in a Pilot-Scale Tubular Photobioreactor

Frontiers in Bioengineering and Biotechnology

Tobias Weise¹  Michael Pfaff³  Claudia Grewe⁴ 

[1] BioControl Jena GmbH, Jena, Germany;Department of Bioinformatics, Friedrich Schiller University Jena, Jena, Germany;Department of Medical Engineering and Biotechnology, University of Applied Sciences Jena, Jena, Germany;Salata AG, Ritschenhausen, Germany;

关键词: microalgal cultivation;    coarse-grained modeling;    light limitation;    temperature;    biomass productivity optimization;    Nannochloropsis;   

DOI  :  10.3389/fbioe.2020.00453

来源: DOAJ

【 摘 要 】

A dynamic coarse-grained model of microalgal growth considering light availability and temperature under discontinuous bioprocess operation was parameterized using experimental data from 15 batch cultivations of Nannochloropsis granulata in a pilot-scale tubular photobioreactor. The methodology applied consists of a consecutive two-step model parameter estimation using pooled, clustered and reorganized data to obtain initial estimates and multi-experiment fitting to obtain the final estimates, which are: maximum specific growth rate μmax = 1.56 d−1, specific photon half-saturation constant KS,ph = 1.89 molphgX-1d-1, specific photon maintenance coefficient mph = 0.346 molphgX-1d-1 and the cardinal temperatures Tmin = 2.3°C, Topt = 27.93°C and Tmax = 32.59°C. Biomass productivity prediction proved highly accurate, expressed by the mean absolute percent error MAPE = 7.2%. Model-based numerical optimization of biomass productivity for repeated discontinuous operation with respect to the process parameters cultivation cycle time, inoculation biomass concentration and temperature yielded productivity gains of up to 35%. This optimization points to best performance under continuous operation. The approach successfully applied here to small pilot-scale confirms an earlier one to lab-scale, indicating its transferability to larger scale tubular photobioreactors.

【 授权许可】

Unknown