Mathematical Description of Functional States in E. coli Fed-batch Cultivation Processes

Bioautomation

关键词: Functional states;    Metabolism;    Mathematical description;    Fed-batch processes;    Escherichia coli;   

DOI  :  

来源: DOAJ

【 摘 要 】

This paper presents an overview of a biochemical correspondence to defined functional states based on specific metabolic mechanisms. For Escherichia coli fed-batch cultivation processes a set of four functional states is considered. It is shown that the process can be divided into several functional states by considering the cell metabolism in more detail. As a result through the proposed functional states the changes in metabolic pathways can be described accurately. For each functional state a respective local model is proposed. Simulations of the E. coli cultivation process with functional state modelling are presented. Furthermore different functional states in a real E. coli MC4110 fed-batch cultivation process are identified and local models are developed. By simulations and comparing results to experimental data is shown that the concept of functional state modelling works in practice and leads to more precise and adequate mathematical description.

【 授权许可】

Unknown