Malaysian Virgin Soil Extracts as Natural Growth Enhancer for Targeted Green Microalgae Species

Applied Sciences

Kazuhiro Komatsu¹  Fridelina Sjahrir²  Emi Fazlina Hashim²  Maegala Nallapan Maniyam³  Hasdianty Abdullah³  Mohd Fadzli Ahmad³  Nor Suhaila Yaacob³  Victor S. Kuwahara⁴ 

[1] Faculty of Education and Graduate School of Engineering Soka University, 1-236 Tangi-Machi, Hachioji-Shi 192-8577, Japan;Faculty of Engineering and Life Sciences, Universiti Selangor, Jalan Timur Tambahan, Bestari Jaya 45600, Malaysia;Institute of Bio-IT Selangor, Universiti Selangor, Jalan Zirkon A7/A, Seksyen 7, Shah Alam 40000, Malaysia;National Institute for Environmental Studies, 16-2 Onogawa, Ibaraki, Tsukuba 305-8506, Japan;

关键词: Chlorella sorokiniana;    microalgae growth;    Nannochloropsis oceanica;    Nannochloropsis oculate;    natural growth nutrient;    virgin soil extract;   

DOI  :  10.3390/app12084060

来源: DOAJ

【 摘 要 】

The microalgae-based industries are trending upwards, particularly as the feed ingredient for aquaculture. Therefore, a sustainable and reasonably priced source of nutrients to support the mass cultivation of microalgae is in great demand. The present study explored the feasibility of using extracts from virgin soil as natural growth-promoting nutrients for the cultivation of Nannochloropsis oculata, Nannochloropsis oceanica, and Chlorella sorokiniana. The extracts were obtained from Bera Lake Forest using five different treatment methods. The greatest retrieval of dissolved organic carbon, total dissolved nitrogen, and total dissolved phosphorus were observed with the autoclave treatment method at 121 °C twice, yielding a respective concentration of 336.56 mg/L, 13.40 mg/L, and 0.14 mg/L, respectively. The highest growth was recorded with Nannochloropsis oculata resulting in an optical density of 0.488 ± 0.009 (×10³ cell mL⁻¹), exhibiting 43% and 44% enhanced growth in comparison to Nannochloropsis oceanica and Chlorella sorokiniana, respectively. The specific growth rate (0.114 ^a ± 0.007 d⁻¹) was the highest for Nannochloropsis oculata when the 24 h-extraction method was used, whereas the utilization of the autoclave 121 °C twice treatment method contributed to the highest specific growth of Nannochloropsis ocenica (0.069 ^a ± 0.003 d⁻¹) and Chlorella sorokiniana (0.080 ^a ± 0.001 d⁻¹). Collectively, these findings suggested that the addition of soil extracts which is sustainable and inexpensive promoted the growth of microalgae compared to the control system. A further study investigating the optimum culture conditions for enhanced microalgae growth will be carried out for the mass production of microalgae biomass.

【 授权许可】

Unknown