Functional Fragments of AIMP1-Derived Peptide (AdP) and Optimized Hydrosol for Their Topical Deposition by Box-Behnken Design

Molecules

Dae-Duk Kim¹  Cheong-Weon Cho²  Young-Min Han²  DongHyun Kim²  Tae-Wan Kwon²  Jae-Young Lee²  Jeong-Jun Lee²  HanSol Lee²  SangKyum Kim²  WooJin Jung²  Jina Kim³  MinChul Park³  Sujin Kang³  Keong-Ryoon Lee⁴ 

[1] College of Pharmacy and Research Institute of Pharmaceutical Sciences, Seoul National University, Seoul 08826, Korea;College of Pharmacy, Chungnam National University, Daejeon 34134, Korea;CureBio Research Center, Suwon 16229, Korea;Laboratory Animal Resource Center, Korea Research Institute of Bioscience and Biotechnology, Ochang 28116, Korea;

关键词: AIMP1-derived peptide (AdP);    cosmeceutical peptide;    Box-Behnken design;    hydrosol;    topical delivery of peptides;   

DOI  :  10.3390/molecules24101967

来源: DOAJ

【 摘 要 】

Aminoacyl-tRNA synthetase complex-interacting multifunctional protein 1 (AIMP1)-derived peptide (AdP) has been developed as a cosmeceutical ingredient for skin anti-aging given its fibroblast-activating (FA) and melanocyte-inhibiting (MI) functions. However, a suitable strategy for the topical delivery of AdP was required due to its low-permeable properties. In this study, FA and MI domains of AdP (FA-AdP and MI-AdP, respectively) were determined by functional domain mapping, where the activities of several fragments of AdP on fibroblast and melanocyte were tested, and a hydrosol-based topical delivery system for these AdP fragments was prepared. The excipient composition of the hydrosol was optimized to maximize the viscosity and drying rate by using Box-Behnken design. The artificial skin deposition of FA-AdP-loaded hydrosol was evaluated using Keshary-Chien diffusion cells equipped with Strat-M membrane (STM). The quantification of the fluorescent dye-tagged FA-AdP in STM was carried out by near-infrared fluorescence imaging. The optimized hydrosol showed 127-fold higher peptide deposition in STM than free FA-AdP (p < 0.05). This work suggests that FA- and MI-AdP are active-domains for anti-wrinkle and whitening activities, respectively, and the hydrosol could be used as a promising cosmetic formulation for the delivery of AdPs to the skin.

【 授权许可】

Unknown