Charge-Sensitive Optical Detection of Binding Kinetics between Phage-Displayed Peptide Ligands and Protein Targets

Biosensors

Guangzhong Ma¹  Runli Liang¹  Shaopeng Wang¹  Yingnan Zhang² 

[1] Biodesign Center for Bioelectronics and Biosensors, Arizona State University, Tempe, AZ 85287, USA;Department of Early Discovery Biochemistry, Genentech, South San Francisco, CA 94080, USA;

关键词: charge-sensitive optical detection;    binding kinetics;    label-free;    phage-display;    peptide;    protein;   

DOI  :  10.3390/bios12060394

来源: DOAJ

【 摘 要 】

Phage display technology has been a powerful tool in peptide drug development. However, the supremacy of phage display-based peptide drug discovery is plagued by the follow-up process of peptides synthesis, which is costly and time consuming, but is necessary for the accurate measurement of binding kinetics in order to properly triage the best peptide leads during the affinity maturation stages. A sensitive technology is needed for directly measuring the binding kinetics of peptides on phages to reduce the time and cost of the entire process. Here, we show the capability of a charge-sensitive optical detection (CSOD) method for the direct quantification of binding kinetics of phage-displayed peptides to their target protein, using whole phages. We anticipate CSOD will contribute to streamline the process of phage display-based drug discovery.

【 授权许可】

Unknown