Male sterile 305 Mutation Leads the Misregulation of Anther Cuticle Formation by Disrupting Lipid Metabolism in Maize

International Journal of Molecular Sciences

Xuejie Yu¹  Qun Sun¹  Ronghuan Gu¹  Yongpei Ke²  Chenxi Feng²  Haichun Shi²  Yang Yu³  Yu Fu³  Jichao Yuan³ 

[1] College of Life Sciences, Sichuan University, Chengdu 610064, China;College of Agronomy, Sichuan Agricultural University, Chengdu 611130, China;Key Laboratory of Bio-resources and Eco-environment, Ministry of Education;

关键词: maize;    ms305;    cuticle;    transcriptomic;    lipidomic;    UHPLC-MS;   

DOI  :  10.3390/ijms21072500

来源: DOAJ

【 摘 要 】

The anther cuticle, which is mainly composed of lipid polymers, functions as physical barriers to protect genetic material intact; however, the mechanism of lipid biosynthesis in maize (Zea mays. L.) anther remains unclear. Herein, we report a male sterile mutant, male sterile 305 (ms305), in maize. It was shown that the mutant displayed a defective anther tapetum development and premature microspore degradation. Three pathways that are associated with the development of male sterile, including phenylpropanoid biosynthesis, biosynthesis of secondary metabolites, as well as cutin, suberine, and wax biosynthesis, were identified by transcriptome analysis. Gas chromatography-mass spectrometry disclosed that the content of cutin in ms305 anther was significantly lower than that of fertile siblings during the abortion stage, so did the total fatty acids, which indicated that ms305 mutation might lead to blocked synthesis of cutin and fatty acids in anther. Lipidome analysis uncovered that the content of phosphatidylcholine, phosphatidylserine, diacylglycerol, monogalactosyldiacylglycerol, and digalactosyldiacylglycerol in ms305 anther was significantly lower when compared with its fertile siblings, which suggested that ms305 mutation disrupted lipid synthesis. In conclusion, our findings indicated that ms305 might affect anther cuticle and microspore development by regulating the temporal progression of the lipidome in maize.

【 授权许可】

Unknown