Comparison of Intestinal Microbiota Between Healthy and MMVD Chihuahuas Using 16S rRNA Gene Amplicon Sequencing

Frontiers in Veterinary Science

Genki Ishihara¹  Mitsuhiro Aizu²  Toshiharu Fukayama²  Mitsuhiro Isaka²  Koji Iwanaga³  Ryuji Araki⁴  Kazunori Ueda⁴  Ayaka Shima⁵ 

[1] Cat Medical Center, Yokohama, Japan;Anicom Insurance, Inc., Tokyo, Japan;Department of Small Animal Clinical Sciences, School of Veterinary Medicine, Rakuno Gakuen University, Ebetsu, Japan;Tokyo Veterinary Cardiology Center, Tokyo, Japan;;Yokohama Yamate Dog &

关键词: Chihuahua;    fractional shortening;    intestinal complication;    intestinal microbiota;    myxomatous mitral valve disease;   

DOI  :  10.3389/fvets.2022.846492

来源: DOAJ

【 摘 要 】

Myxomatous mitral valve disease (MMVD) is the most common cause of congestive heart failure in dogs, and although complications of MMVD to the lungs and kidneys have been identified, complications to the gut are less well understood. The intestinal microbiota is an important factor in the gut, and although the association between heart disease and the intestinal microbiota has been shown in human medicine, it is unknown in dogs. The study aimed to evaluate the relationship between MMVD and gut microbiota. A total of 69 healthy Chihuahuas and Chihuahuas with MMVD were evaluated for cardiac health by echocardiography and chest radiography and grouped according to ACVIM guidelines. Fecal samples were collected from all cases and 16S rRNA sequencing was used to reveal the intestinal microbiota. There were significant differences in LA/Ao, LVIDd, E vel, VHS, and VLAS with the severity of ACVIM. On the other hand, there were no significant differences in the diversity and composition of gut microbiota among the groups. The present study did not identify the effects of MMVD on the gut microbiota.

【 授权许可】

Unknown