Comparative Analysis of Genome Editors Efficiency on a Model of Mice Zygotes Microinjection

International Journal of Molecular Sciences

Olga A. Dontsova¹  Petr V. Sergiev²  Olga O. Grigorieva²  Oleg A. Permyakov²  Olga A. Averina²  Alexey S. Starshin³  Alexander M. Mazur³  Egor B. Prokhortchouk³ 

[1] Department of Chemistry, Lomonosov Moscow State University, 119991 Moscow, Russia;Institute of Functional Genomics, Lomonosov Moscow State University, 119991 Moscow, Russia;Research Center of Biotechnology of the Russian Academy of Sciences, Institute of Bioengineering, 119071 Moscow, Russia;

关键词: genome editing;    CRISPR Cas9;    knockout mice;    microinjection;    homologous recombination;    prime editor;   

DOI  :  10.3390/ijms221910221

来源: DOAJ

【 摘 要 】

Genome editing is an indispensable tool for functional genomics. The caveat of the genome-editing pipeline is a prevalence of error-prone non-homologous end joining over homologous recombination, while only the latter is suitable to introduce particularly desired genetic variants. To overcome this problem, a toolbox of genome engineering was appended by a variety of improved instruments. In this work, we compared the efficiency of a number of recently suggested improved systems for genome editing applied to the same genome regions on a murine zygote model via microinjection. As a result, we observed that homologous recombination utilizing an ssDNA template following sgRNA directed Cas9 cleavage is still the method of choice for the creation of animals with precise genome alterations.

【 授权许可】

Unknown