Investigating Transcriptional Dynamics Changes and Time-Dependent Marker Gene Expression in the Early Period After Skeletal Muscle Injury in Rats

Frontiers in Genetics

Kang Ren¹  Guoshuai An²  Yingjie Tian²  Qiuxiang Du²  Liangliang Wang²  Liang Wang²  Yingyuan Wang²  Junhong Sun²  Qianqian Jin²  Jie Cao²  Na Li²  Lihong Dang² 

[1] Department of Basic Medicine, Changzhi Medical College, Changzhi, China;School of Forensic Medicine, Shanxi Medical University, Jinzhong, China;

关键词: time-series RNA-seq;    gene expression and regulation landscape;    time-related biomarkers;    transcriptional dynamics;    skeletal muscle injury;   

DOI  :  10.3389/fgene.2021.650874

来源: DOAJ

【 摘 要 】

Following skeletal muscle injury (SMI), from post-injury reaction to repair consists of a complex series of dynamic changes. However, there is a paucity of research on detailed transcriptional dynamics and time-dependent marker gene expression in the early stages after SMI. In this study, skeletal muscle tissue in rats was taken at 4 to 48 h after injury for next-generation sequencing. We examined the transcriptional kinetics characteristics during above time periods after injury. STEM and maSigPro were used to screen time-correlated genes. Integrating 188 time-correlated genes with 161 genes in each time-related gene module by WGCNA, we finally identified 18 network-node regulatory genes after SMI. Histological staining analyses confirmed the mechanisms underlying changes in the tissue damage to repair process. Our research linked a variety of dynamic biological processes with specific time periods and provided insight into the characteristics of transcriptional dynamics, as well as screened time-related biological indicators with biological significance in the early stages after SMI.

【 授权许可】

Unknown