Redox Proteomics and Platelet Activation: Understanding the Redox Proteome to Improve Platelet Quality for Transfusion

International Journal of Molecular Sciences

Jean-Daniel Tissot¹  Niels Lion¹  Michel Prudent¹  Giona Sonego¹  Mélanie Abonnenc¹ 

[1] Transfusion Interrégionale Croix Rouge Suisse SA, Laboratoire de Recherche sur les Produits Sanguins, Route de la Corniche 2, 1066 Epalinges, Switzerland;

关键词: activation;    aggregation;    blood products;    phosphorylation;    platelet function;    platelets;    reactive oxygen species;    redox cysteine;    transfusion;   

DOI  :  10.3390/ijms18020387

来源: DOAJ

【 摘 要 】

Blood banks use pathogen inactivation (PI) technologies to increase the safety of platelet concentrates (PCs). The characteristics of PI-treated PCs slightly differ from those of untreated PCs, but the underlying reasons are not well understood. One possible cause is the generation of oxidative stress during the PI process. This is of great interest since reactive oxygen species (ROS) act as second messengers in platelet functions. Furthermore, there are links between protein oxidation and phosphorylation, another mechanism that is critical for cell regulation. Current research efforts focus on understanding the underlying mechanisms and identifying new target proteins. Proteomics technologies represent powerful tools for investigating signaling pathways involving ROS and post-translational modifications such as phosphorylation, while quantitative techniques enable the comparison of the platelet resting state versus the stimulated state. In particular, redox cysteine is a key player in platelet activation upon stimulation by different agonists. This review highlights the experiments that have provided insights into the roles of ROS in platelet function and the implications for platelet transfusion, and potentially in diseases such as inflammation and platelet hyperactivity. The review also describes the implication of redox mechanism in platelet storage considerations.

【 授权许可】

Unknown