Identification of differentially expressed genes in sunflower (Helianthus annuus) leaves and roots under drought stress by RNA sequencing

Botanical Studies

Weizhong Li¹  Chunbo Liang²  Xutang Huang²  Shuquan Zhang³  Liguo Zhang³  Cen Li³  Ying Yu³  Fei Zhou³  Jun Ma³  Jing Wang³  Wenjun Wang³ 

[1] Crop Research and Breeding Center of Land-Reclamation of Heilongjiang Province;Heilongjiang Academy of Agricultural Sciences Postdoctoral Programme;Institute of Industrial Crops, Heilongjiang Academy of Agricultural Sciences;

关键词: Digital gene expression;    Drought stress;    Leaf;    Sunflower (Helianthus annuus L.);    Root;   

DOI  :  10.1186/s40529-017-0197-3

来源: DOAJ

【 摘 要 】

Abstract Background Sunflower is recognized as one of the most important oil plants with strong tolerance to drought in the world. In order to study the response mechanisms of sunflower plants to drought stress, gene expression profiling using high throughput sequencing was performed for seedling leaves and roots (sunflower inbred line R5) after 24 h of drought stress (15% PEG 6000). The transcriptome assembled using sequences of 12 samples was used as a reference. Results 805 and 198 genes were identified that were differentially expressed in leaves and roots, respectively. Another 71 genes were differentially expressed in both organs, in which more genes were up-regulated than down-regulated. In agreement with results obtained for other crops or from previous sunflower studies, we also observed that nine genes may be associated with the response of sunflower to drought. Conclusions The results of this study may provide new information regarding the sunflower drought response, as well as add to the number of known genes associated with drought tolerance.

【 授权许可】

Unknown