Finding Species-Specific Extracellular Surface-Facing Proteomes in Toxic Dinoflagellates

Toxins

Kenrick Kai-yuen Chan¹  Sirius Pui-kam Tse¹  Zoe Chan¹  Kevin W. H. Kwok¹  Samuel Chun-lap Lo¹  Hang-kin Kong¹  Pak-yeung Lo¹ 

[1] Department of Applied Biology and Chemical Technology, Faculty of Applied Science and Textiles, The Hong Kong Polytechnic University, Hung Hom, Hong Kong;

关键词: proteomics;    toxic dinoflagellates;    cell surface labeling;    orthogroup inference;   

DOI  :  10.3390/toxins13090624

来源: DOAJ

【 摘 要 】

As a sequel to our previous report of the existence of species-specific protein/peptide expression profiles (PEPs) acquired by mass spectrometry in some dinoflagellates, we established, with the help of a plasma-membrane-impermeable labeling agent, a surface amphiesmal protein extraction method (SAPE) to label and capture species-specific surface proteins (SSSPs) as well as saxitoxins-producing-species-specific surface proteins (Stx-SSPs) that face the extracellular space (i.e., SSSPs_Ef and Stx-SSPs_Ef). Five selected toxic dinoflagellates, Alexandrium minutum, A. lusitanicum, A. tamarense, Gymnodinium catenatum, and Karenia mikimotoi, were used in this study. Transcriptomic databases of these five species were also constructed. With the aid of liquid chromatography linked-tandem mass spectrometry (LC-MS/MS) and the transcriptomic databases of these species, extracellularly facing membrane proteomes of the five different species were identified. Within these proteomes, 16 extracellular-facing and functionally significant transport proteins were found. Furthermore, 10 SSSPs and 6 Stx-SSPs were identified as amphiesmal proteins but not facing outward to the extracellular environment. We also found SSSPs_Ef and Stx-SSPs_Ef in the proteomes. The potential functional correlation of these proteins towards the production of saxitoxins in dinoflagellates and the degree of species specificity were discussed accordingly.

【 授权许可】

Unknown