Solid-State Fermentation with Aspergillus niger GH1 to Enhance Polyphenolic Content and Antioxidative Activity of Castilla Rose (Purshia plicata)

Plants

DeLeón-Medina José Carlos¹  Sepúlveda Leonardo¹  Ascacio-Valdés Juan¹  Aguilar Cristóbal Noé¹  Morlett-Chávez Jesús²  Zugasti-Cruz Alejandro³  Meléndez-Renteria Paola⁴ 

[1] Bioprocesses and Bioproducts Research Group, Food Research Department, School of Chemistry, Autonomous University of Coahuila, Saltillo 25280, Mexico;Laboratory of Molecular Biology, School of Chemistry, Autonomous University of Coahuila, Saltillo 25280, Mexico;Laboratory of Toxicology, School of Chemistry, Autonomous University of Coahuila, Saltillo 25280, Mexico;Research and Conservation Center of Coahuila Biodiversity and Ecology, Autonomous University of Coahuila, Cuatrociénegas 27640, Mexico;

关键词: antioxidant activity;    Aspergillus niger GH1;    Castilla Rose;    polyphenols;    solid-state fermentation;   

DOI  :  10.3390/plants9111518

来源: DOAJ

【 摘 要 】

This work was performed to study Castilla Rose (Purshia plicata) as a potential source of polyphenols obtained by solid-state fermentation (SSF)-assisted extraction using the microorganism Aspergillus niger GH1 and to evaluate the antioxidant activity of the extracted compounds. First, water absorption capacity (WAC) of the plant material, radial growth of the microorganism, determination of best fermentation conditions, and maximum accumulation time of polyphenols were tested. Then, a larger-scale fermentation, polyphenols isolation by column liquid chromatography (Amberlite XAD-16) and recovered compounds identification by HPLC-MS were made. Finally, the antioxidant activity of the recovered compounds was tested by ABTS, DPPH, and lipid oxidation inhibition assays. The best fermentation conditions were temperature 25 °C and inoculum 2 × 10⁶ spores/g, while the maximum extraction time of polyphenols was 24 h (173.95 mg/g). The HPLC/MS analysis allowed the identification of 25 different polyphenolic compounds, and the antioxidant activity of the obtained polyphenols was demonstrated, showing ABTS assay the most effective with inhibition of 94.34%.

【 授权许可】

Unknown