Recovery of Low-molecular Weight Galloyltannins from Agricultural Residue of Juglans sigillata Dode Seed Husks and their Tyrosinase Inhibitory Effect

BioResources

Ting Shen¹  Wei-Cheng Hu¹  Xue-Gang Luo²  Chuan-Ling Si³  Lei Wu⁴  Xiao-Dan Ren⁴  Xiao-Feng Huang⁴  Zhen-Guo Du⁴ 

[1] Jiangsu Key Laboratory for Eco-Agricultural Biotechnology around Hongze Lake, Huaiyin Normal University, Huaian 223300, China;Key Laboratory of Industrial Fermentation Microbiology of Ministry of Education, and Tianjin Key Laboratory of Industrial Microbiology, Tianjin University of Science & Technology, Tianjin 300457, China;Tianjin Key Lab of Pulp & Paper, College of Materials Science & Chemical Engineering, Tianjin University of Science & Technology. No.29 at 13th Avenue of TEDA, Tianjin 300457, P.R. China;Tianjin Key Laboratory of Pulp & Paper, Tianjin University of Science & Technology, Tianjin 300457, China;

关键词: Juglans sigillata;    Extractives;    Galloyltannin;    Tyrosinase;    B16F10 mouse melanoma cell;   

DOI  :  10.15376/biores.9.2.2226-2236

来源: DOAJ

【 摘 要 】

The phytochemical investigation, including fractionation and purification of 70% acetone extracts of Juglans sigillata seed husks, an agricultural residue, led to the isolation of five low-molecular weight galloyltannins. The structures of the extractives were elucidated as 1,2,6-tri-O-galloyl-β-D-glucose (1), 3,4,6-tri-O-galloyl-β-D-glucose (2), 2,3,4,6-tetra-O-galloyl-β-D-glucose (3), 1,2,3,4,6-penta-O-galloyl-β-D-glucose (4), and tannic acid (5), primarily based on their spectral (NMR and MS) and chemical evidence. Galloyltannins 1-5 showed strong inhibitory activity against mushroom tyrosinase, with IC50 values ranging from 35.27 to 76.37 μM; kojic acid, which was used as a positive control, had an IC50 value of 342.14 μM. It was further found that 1-5 inhibited melanin production and exhibited intracellular tyrosinase activity, as well as down-regulated mRNA and protein expression levels of tyrosinase, in B16F10 mouse melanoma cells. Therefore, the isolated extractives from seed husks of J. sigillata may serve as potential candidates for hyperpigmentation remediation and as skin-whitening agents in the cosmetics industry.

【 授权许可】

Unknown