Synaptic transmission parallels neuromodulation in a central food-intake circuit

eLife

Andreas Schoofs¹  Anton Miroschnikow¹  Sebastian Hückesfeld¹  Philipp Schlegel¹  Michael J Pankratz¹  Marc Peters¹  James W Truman²  Michael J Texada²  Albert Cardona²  Casey M Schneider-Mizell²  Feng Li²  Haluk Lacin²  Richard D Fetter² 

[1] Department of Molecular Brain Physiology and Behavior, LIMES Institute, University of Bonn, Bonn, Germany;Janelia Research Campus, Howard Hughes Medical Institute, Ashburn, United States;

关键词: acetylcholine;    neuropeptides;    endocrine;    co-transmission;    neuromedin;    hugin;   

DOI  :  10.7554/eLife.16799

来源: DOAJ

【 摘 要 】

NeuromedinU is a potent regulator of food intake and activity in mammals. In Drosophila, neurons producing the homologous neuropeptide hugin regulate feeding and locomotion in a similar manner. Here, we use EM-based reconstruction to generate the entire connectome of hugin-producing neurons in the Drosophila larval CNS. We demonstrate that hugin neurons use synaptic transmission in addition to peptidergic neuromodulation and identify acetylcholine as a key transmitter. Hugin neuropeptide and acetylcholine are both necessary for the regulatory effect on feeding. We further show that subtypes of hugin neurons connect chemosensory to endocrine system by combinations of synaptic and peptide-receptor connections. Targets include endocrine neurons producing DH44, a CRH-like peptide, and insulin-like peptides. Homologs of these peptides are likewise downstream of neuromedinU, revealing striking parallels in flies and mammals. We propose that hugin neurons are part of an ancient physiological control system that has been conserved at functional and molecular level.

【 授权许可】

Unknown