Apolipoprotein A1-Unique Peptide as a Diagnostic Biomarker for Acute Ischemic Stroke

International Journal of Molecular Sciences

Guangyu Li¹  Lei Dong¹  Wenlong Xu¹  Xu Zhao²  Yuan Wang²  Bing Gao²  Yue Yu²  Wentao Zhang² 

[1] Department of Neurosurgery, First Affiliated Hospital of China Medical University, Shenyang 110001, China;School of Bioscience and Bioengineering, South China University of Technology, Guangzhou 510006, China;

关键词: apolipoprotein A1 unique peptide (APOA1-UP);    acute ischemic stroke (AIS);    biomarker;    high-density lipoprotein cholesterol (HDL-C);    multiple reaction monitoring (MRM);    labeled reference peptide (LRP);   

DOI  :  10.3390/ijms17040458

来源: DOAJ

【 摘 要 】

Clinically-informative biomarkers of ischemic stroke are needed for rapid diagnosis and timely treatment. In the present study, APOA1 unique peptide (APOA1-UP), a novel peptide biomarker, was identified and quantified by multiple reaction monitoring (MRM) using labeled reference peptide (LRP). Serum samples of 94 patients in the ischemic stroke group and 37 patients in the non-stroke group were analyzed for the levels of total APOA1-UP, low density lipoprotein cholesterol (LDL-C), triglycerides (TG), high-density lipoprotein cholesterol (HDL-C), and total cholesterol (TC). Median ratio of total APOA1-UP/LRP was 2.14 (interquartile range, 0.40) in the non-stroke group and 1.32 (0.44) in the ischemic stroke group (p < 0.0001). The serum level of total APOA1-UP was independently correlated with the presence of ischemic stroke by multivariate logistic regression analysis (p < 0.0001). From the receiver operating characteristic (ROC) curve, the area under the curve (AUC) was 0.9750 and the optimal cutoff value of the serum APOA1-UP level was 1.80, which yielded a sensitivity of 90.63% and a specificity of 97.14%. The diagnostic efficiency of HDL-C was lower, with an AUC of 0.7488. Therefore, the serum level of APOA1-UP is a diagnostic biomarker candidate for ischemic stroke in the early stage.

【 授权许可】

Unknown