Label-Free Morpho-Molecular Imaging for Studying the Differential Interaction of Black Phosphorus with Tumor Cells

Nanomaterials

Debadrita Paria¹  Ishan Barman¹  Annalisa Convertino²  Sara De Simone²  Valentina Mussi²  Manuel Serrano-Ruiz³  Maria Caporali³  Ines Fasolino⁴  Luigi Ambrosio⁴  Maria Grazia Raucci⁴ 

[1] Department of Mechanical Engineering, Johns Hopkins University, Baltimore, MD 21218, USA;Institute for Microelectronics and Microsystems, National Research Council, 00133 Rome, Italy;Institute for the Chemistry of Organometallic Compounds, National Research Council, 50019 Sesto Fiorentino, Italy;Institute of Polymers, Composites and Biomaterials, National Research Council, 80125 Naples, Italy;

关键词: black phosphorus;    optical diffraction tomography;    Raman mapping;    cellular internalization;   

DOI  :  10.3390/nano12121994

来源: DOAJ

【 摘 要 】

Black phosphorus nanosheets (2D BP) are emerging as very promising, highly selective chemotherapeutic agents due to their fast degradation in the intracellular matrix of cancer cells. Here, optical diffraction tomography (ODT) and Raman spectroscopy were exploited as a powerful label-free approach to achieve integrated insights into the processes accompanying the administration of exfoliated 2D BP flakes in human prostatic adenocarcinoma and normal human prostate epithelial cells. Our ODT experiments provided unambiguous visualization of the 2D BP internalization in cancer cells and the morphological modifications of those cells in the apoptotic phase. The cellular internalization and damaging occurred, respectively, 18 h and 36–48 h after the 2D BP administration. Changes in the chemical properties of the internalized 2D BP flakes were monitored by Raman spectroscopy. Interestingly, a fast oxidation process of the 2D BP flakes was activated in the intracellular matrix of the cancer cells after 24 h of incubation. This was in sharp contrast to the low 2D BP uptake and minimal chemical changes observed in the normal cells. Along with the understanding of the 2D BP fate in the cancer cells, the proposed label-free morpho-molecular approach offers a powerful, rapid tool to study the pharmacokinetic properties of engineered nanomaterials in preclinical research.

【 授权许可】

Unknown