Evaluation of Antiradical and Antioxidant Activities of Lipopeptides Produced by Bacillus subtilis Strains

Frontiers in Microbiology

Alexandre Noblecourt¹  François Coutte¹  Chloé Dupuis²  Rozenn Ravallec³  Elodie Dussert³  Christophe Flahaut³  Mélissa Tourret³  Josette Behra-Miellet³ 

[1] LIPOFABRIK, Lesquin, France;Univ. Lille, UMRT 1158 BioEcoAgro - Métabolites secondaires d'origine microbienne, Institut Charles Viollette, Lille, France;Univ. Lille, Univ. Artois, UMRT 1158 BioEcoAgro - Bénéfice santé d'hydrolysats de protéines et coproduits agro-alimentaires, Institut Charles Viollette, Lille, France;

关键词: antioxidant;    antiradical;    Bacillus subtilis;    lipopeptides;    reactive oxygen species;   

DOI  :  10.3389/fmicb.2022.914713

来源: DOAJ

【 摘 要 】

This study investigated the antiradical and antioxidant potential of the three families of lipopeptides (i.e., surfactin, mycosubtilin, and plipastatin/fengycin) produced by Bacillus subtilis strains. The antiradical/antioxidant activities of highly purified lipopeptides were studied in acellular models using a 2,2-diphenyl-1-picrylhydrazyl (DPPH) radical, superoxide anion (O2.-), hydrogen peroxide, (H2O2) and hydroxyl radical (HO.). At a lipopeptide concentration of 500 mg.L−1, the maximum inhibition of DPPH reached 22.88% (obtained for plipastatin). Moreover, the scavenging effects of O2.-, H2O2, and HO. at the highest concentration tested (250 mg.L−1) were found to be 6, 21, and 3% for surfactin, 19, 9, and 15% for mycosubtilin, 21, 18, and 59% for plipastatin, 21, 31, and 61% for the mixture of surfactin/plipastatin, and 13, 16, and 15% for the mixture of surfactin/mycosubtilin, respectively. These results showed that plipastatin was the best candidate due to its antioxidant activities.

【 授权许可】

Unknown