Emergence of NDM-1- and CTX-M-3-Producing Raoultella ornithinolytica in Human Gut Microbiota

Frontiers in Microbiology

Shuang Wang¹  Zengqiang Kou¹  Peibin Hou¹  Liuchen Xu¹  Yan Li¹  Beiwen Zheng²  Xiaohui Chi³  Hengjie Xie⁴  Zhenwang Bi⁵ 

[1] Bacterial Infection Disease Control of Institute, Shandong Center for Disease Control and Prevention, Jinan, China;Collaborative Innovation Center for Diagnosis and Treatment of Infectious Diseases, State Key Laboratory for Diagnosis and Treatment of Infectious Diseases, The First Affiliated Hospital, College of Medicine, Zhejiang University, Hangzhou, China;Department of Environment and Health, School of Public Health, Shandong University, Jinan, China;Department of Supervise Sampling, Shandong Institute for Food and Drug Control, Jinan, China;Shandong Academy of Clinical Medicine, Shandong Provincial Hospital, Jinan, China;

关键词: Raoultella ornithinolytica;    carbapenemase;    extended-spectrum β-lactamase;    blaNDM-1;    blaCTX-M;    whole-genome sequencing;   

DOI  :  10.3389/fmicb.2019.02678

来源: DOAJ

【 摘 要 】

Raoultella ornithinolytica is an opportunistic pathogen of the Enterobacteriaceae family and has been implicated in nosocomial infections in recent years. The aim of this study was to characterize a carbapenemase-producing R. ornithinolytica isolate and three extended-spectrum β-lactamase (ESBL)-producing R. ornithinolytica isolates from stool samples of adults in a rural area of Shandong Province, China. The species were identified using matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF MS) and 16S rDNA sequence analysis. Antimicrobial susceptibility test showed that all four isolates were multidrug-resistant (MDR). The whole genome sequence (WGS) of these isolates was determined using an Illumina HiSeq platform, which revealed MDR-related genes. The S1 nuclease-pulsed-field gel electrophoresis (S1-PFGE) was used to characterize the plasmids carried by the R. ornithinolytica isolates. The blaNDM-1 and blaCTX-M-3 genes were probed using Southern blotting, which confirmed the location of both genes on the same plasmid with molecular weight of 336.5–398.4 kb. The transferability of blaNDM-1 and blaCTX-M was also confirmed by conjugation assays. Finally, BLAST analysis of both genes showed that mobile genetic elements were associated with the spread of drug resistance genes. Taken together, we report the presence of conjugative blaNDM-1 and blaCTX-M plasmids in R. ornithinolytica isolates from healthy humans, which indicate the possibility of inter-species transfer of drug resistance genes. To the best of our knowledge, this is the first study to isolate and characterize carbapenemase-producing R. ornithinolytica and ESBL-producing R. ornithinolytica isolates from healthy human hosts.

【 授权许可】

Unknown