12/111phiA Prophage Domestication Is Associated with Autoaggregation and Increased Ability to Produce Biofilm in Streptococcus agalactiae

Microorganisms

SeydinaM. Diene¹  Patrice Francois²  JulienBurlaud Gaillard³  Houssein Gbaguidi-Haore⁴  Luka Courtier-Martinez⁵  Roland Quentin⁵  Laurent Mereghetti⁵  Nathalie Van Der Mee-Marquet⁵  Adélaïde Renard⁵ 

[1] Aix-Marseille Université, MEPHI, IRD, APHM, IHU-Méditerranée Infection, Faculté de Pharmacie, 13000 Marseille, France;Laboratoire de Recherche Génomique, Service des Maladies Infectieuses, Centre Médical Universitaire, Hôpitaux Universitaire de Genève, 1205 Geneva, Switzerland;Plateforme IBiSA Microscopie Electronique, Faculté de Médecine, Université de Tours, 37000 Tours, France;Service d’Hygiène Hospitalière, CHRU, 25056 Besançon, France;UMR INRAE 1282 Infectiologie et Santé Publique, Bactéries et Risque Materno-Foetal, Université de Tours, 37000 Tours, France;

关键词: Streptococcus agalactiae;    phage;    autoaggregation;    biofilm;    pathogenicity;    neonate;   

DOI  :  10.3390/microorganisms9061112

来源: DOAJ

【 摘 要 】

CC17 Streptococcus agalactiae carrying group-A prophages is increasingly responsible for neonatal infections. To investigate the impact of the genetic features of a group-A prophage, we first conducted an in silico analysis of the genome of 12/111phiA, a group-A prophage carried by a strain responsible for a bloodstream infection in a parturient. This revealed a Restriction Modification system, suggesting a prophage maintenance strategy and five ORFs of interest for the host and encoding a type II toxin antitoxin system RelB/YafQ, an endonuclease, an S-adenosylmethionine synthetase MetK, and an StrP-like adhesin. Using the WT strain cured from 12/111phiA and constructing deleted mutants for the ORFs of interest, and their complemented mutants, we demonstrated an impact of prophage features on growth characteristics, cell morphology and biofilm formation. Our findings argue in favor of 12/111phiA domestication by the host and a role of prophage features in cell autoaggregation, glycocalyx and biofilm formation. We suggest that lysogeny may promote GBS adaptation to the acid environment of the vagina, consequently colonizing and infecting neonates.

【 授权许可】

Unknown