Genome-Wide Identification and Expression Analysis of SNARE Genes in Brassica napus

Plants

Yakubu Saddeeq Abubakar¹  Jiandong Bao¹  Jing Xu²  Xu Zhao²  Zonghua Wang²  Mengjiao Zhang²  Yanan Shen²  Yanan Shan²  Guodong Lu²  Airong Wang² 

[1] College of Life Science, Fujian Agriculture and Forestry University, Fuzhou 350002, China;State Key Laboratory of Ecological Pest Control for Fujian and Taiwan Crops, Fujian Agriculture and Forestry University, Fuzhou 350002, China;

关键词: Brassica napus;    SNARE;    gene family;    Sclerotinia sclerotiorum;    expression profile;    hormone stimuli;   

DOI  :  10.3390/plants11050711

来源: DOAJ

【 摘 要 】

SNAREs (soluble N-ethylmaleimide-sensitive factor attachment protein receptors) are central components that drive membrane fusion events during exocytosis and endocytosis and play important roles in different biological processes of plants. In this study, we identified 237 genes encoding SNARE family proteins in B. napus in silico at the whole-genome level. Phylogenetic analysis showed that BnaSNAREs could be classified into five groups (Q (a-, b-, c-, bc-) and R) like other plant SNAREs and clustered into twenty-five subclades. The gene structure and protein domain of each subclade were found to be highly conserved. In many subclades, BnaSNAREs are significantly expanded compared with the orthologous genes in Arabidopsis thaliana. BnaSNARE genes are expressed differentially in the leaves and roots of B. napus. RNA-seq data and RT-qPCR proved that some of the BnaSNAREs are involved in the plant response to S. sclerotiorum infection as well as treatments with toxin oxalic acid (OA) (a virulence factor often secreted by S. sclerotiorum) or abscisic acid (ABA), methyl jasmonate (MeJA), and salicylic acid (SA), which individually promote resistance to S. sclerotiorum. Moreover, the interacted proteins of BnaSNAREs contain some defense response-related proteins, which increases the evidence that BnaSNAREs are involved in plant immunity. We also found the co-expression of BnaSYP121/2s, BnaSNAPs, and BnaVAMP722/3s in B. napus due to S. sclerotiorum infection as well as the probable interaction among them.

【 授权许可】

Unknown