Regional differences in the number and type of myenteric neurons in the descending colon of rats

Arquivos de Neuro-Psiquiatria

Sônia Lucy Molinari¹  Marcílio Hubner de Miranda Neto¹  Eduardo José de Almeida Araújo²  Débora de Mello Gonçales Sant'Ana² 

[1] Universidade Estadual de Maringá;Universidade Paranaense;

关键词: myenteric plexus;    Giemsa;    NADH-diaphorase;    NADPH-diaphorase;    rat;    descending colon;   

DOI  :  10.1590/S0004-282X2003000200011

来源: DOAJ

【 摘 要 】

The purpose of this study was to analyze the neuronal density of the myenteric plexus of the intermediate and antimesocolic regions of the descending colon of rats. Whole-mounts were stained with three different techniques of neuronal evidenciation. Through counts of the number of neurons in an area of 6.64 mm² under light microscopy, we found 1,271 ± 227.54 neurons with Giemsa in the intermediate region and 1,234 ± 225.92 neurons in the antimesocolic region; with the NADH-diaphorase technique we found 530 ± 92.97 neurons in the intermediate region and 539 ± 146.72 neurons in the antimesocolic region; and through the NADPH-diaphorase histochemistry, we found 417 ± 34.42 neurons in the intermediate region and 547 ± 84.01 neurons in the antimesocolic region. We conclude that there is a variation in the density of NADPH-diaphorase positive neurons in the intestinal circumference; that the NADH-diaphorase positive neuronal subpopulation represented 42.7% of that stained with Giemsa; and that the NADPH-diaphorase positive neurons represented 37.8% of the whole myenteric population.

【 授权许可】

Unknown