Molecular Cloning and Expression Profile of Class E Genes Related to Sepal Development in Nelumbo nucifera

Plants

Weiwei Zhang¹  Lijie Cui²  Fengluan Liu²  Daike Tian³  Hanchun Li³  Qingqing Liu³  Dasheng Zhang³  Xiangjie An³  Zhuoxing Liu³  Lei Xiong³ 

[1] Department of Plant Science and Technology, Shanghai Vocational College of Agriculture and Forestry, Shanghai 201699, China;Development Center of Plant Germplam Resources, College of Life Science, Shanghai Normal University, Shanghai 200234, China;Shanghai Key Laboratory of Plant Functional Genomics and Resources, Shanghai Chenshan Plant Science Research Center of Chinese Academy of Sciences, Shanghai Chenshan Botanical Garden, Shanghai 201602, China;

关键词: MADS-box;    SEPALLATA;    AGL9;    MADS6-like;    sepal;    petal;   

DOI  :  10.3390/plants10081629

来源: DOAJ

【 摘 要 】

The lotus (Nelumbo Adans.) is an important aquatic plant with ornamental, medicinal and edible values and cultural connotations. It has single-, semi-double-, double- and thousand-petalled types of flower shape and is an ideal material for developmental research of flower doubling. The lotus is a basal eudicot species without a morphological difference between the sepals and petals and occupies a critical phylogenetic position in flowering plants. In order to investigate the genetic relationship between the sepals and petals in the lotus, the class E genes which affect sepal formation were focused on and analyzed. Here, SEPALLATA 1(NnSEP1) and its homologous genes AGAMOUS-LIKE MADS-BOXAGL9 (NnAGL9) and MADS-BOX TRANSCRIPTION FACTOR 6-like (NnMADS6-like) of the class E gene family were isolated from the flower buds of the Asian lotus (Nelumbo nucifera Gaertn.). The protein structure, subcellular localization and expression patterns of these three genes were investigated. All three genes were verified to locate in the nucleus and had typical MADS-box characteristics. NnSEP1 and NnMADS6-like were specifically expressed in the sepals, while NnAGL9 was highly expressed in the petals, suggesting that different developmental mechanisms exist in the formation of the sepals and petals in the lotus. The significant functional differences between NnSEP1, NnMADS6-like and NnAGL9 were also confirmed by a yeast two-hybrid assay. These results expand our knowledge on the class E gene family in sepal formation and will benefit fundamental research on the development of floral organs in Nelumbo.

【 授权许可】

Unknown