Endogenous Phenolics in Hulls and Cotyledonsof Mustard and Canola: A Comparative Study onIts Sinapates and Antioxidant Capacity

Antioxidants

Ayyappan Aachary¹  Shyamchand Mayengbam¹  Usha Thiyam-Holländer¹ 

[1] Department of Human Nutritional Sciences, University of Manitoba, Winnipeg,MB R3T 2N2, Canada;

关键词: sinapic acid derivatives;    sinapine;    sinapoyl glucose;    mustard;    canola;    hulls;    cotyledons;    antioxidant activity;   

DOI  :  10.3390/antiox3030544

来源: DOAJ

【 摘 要 】

Endogenous sinapic acid (SA), sinapine (SP), sinapoyl glucose (SG) and canolol (CAN) of canola and mustard seeds are the potent antioxidants in various lipid-containing systems. The study investigated these phenolic antioxidants using different fractions of canola and mustard seeds. Phenolic compounds were extracted from whole seeds and their fractions: hulls and cotyledons, using 70% methanol by the ultrasonication method and quantified using HPLC-DAD. The major phenolics from both hulls and cotyledons extracts were SP, with small amounts of SG, and SA with a significant difference of phenolic contents between the two seed fractions. Cotyledons showed relatively high content of SP, SA, SG and total phenolics in comparison to hulls (p < 0.001). The concentration of SP in different fractions ranged from 1.15 ± 0.07 to 12.20 ± 1.16 mg/g and followed a decreasing trend- canola cotyledons > mustard cotyledons > mustard seeds > canola seeds > mustard hulls > canola hulls. UPLC-tandem Mass Spectrometry confirmed the presence of sinapates and its fragmentation in these extracts. Further, a high degree of correlation(r = 0.93) was noted between DPPH scavenging activity and total phenolic content.

【 授权许可】

Unknown