Characterizing the Potential of the Non-Conventional Yeast Saccharomycodes ludwigii UTAD17 in Winemaking

Microorganisms

Nuno Pereira Mira¹  Isabel Vasconcelos¹  Arlete Mendes-Faia²  Catarina Barbosa²  Ana Mendes-Ferreira³  MariaJoão Tavares⁴  Marcos Esteves⁴ 

[1] Biotechnology, Department of Biology and Environment, Universidade de Trás-os-Montes e Alto Douro, 5000-801 Vila Real, Portugal;;B—Laboratory of Wine Microbiology &CBQF/Centro de Biotecnologia e Química Fina, Escola Superior de Biotecnologia, Universidade Católica Portuguesa, 4169-005 Porto, Portugal;;WM&

关键词: saccharomycodes ludwigii;    non-saccharomyces;    wine fermentation;    mixed-culture;    wine aroma;   

DOI  :  10.3390/microorganisms7110478

来源: DOAJ

【 摘 要 】

Non-Saccharomyces yeasts have received increased attention by researchers and winemakers, due to their particular contributions to the characteristics of wine. In this group, Saccharomycodes ludwigii is one of the less studied species. In the present study, a native S. ludwigii strain, UTAD17 isolated from the Douro wine region was characterized for relevant oenological traits. The genome of UTAD17 was recently sequenced. Its potential use in winemaking was further evaluated by conducting grape-juice fermentations, either in single or in mixed-cultures, with Saccharomyces cerevisiae, following two inoculation strategies (simultaneous and sequential). In a pure culture, S. ludwigii UTAD17 was able to ferment all sugars in a reasonable time without impairing the wine quality, producing low levels of acetic acid and ethyl acetate. The overall effects of S. ludwigii UTAD17 in a mixed-culture fermentation were highly dependent on the inoculation strategy which dictated the dominance of each yeast strain. Wines whose fermentation was governed by S. ludwigii UTAD17 presented low levels of secondary aroma compounds and were chemically distinct from those fermented by S. cerevisiae. Based on these results, a future use of this non-Saccharomyces yeast either in monoculture fermentations or as a co-starter culture with S. cerevisiae for the production of wines with greater expression of the grape varietal character and with flavor diversity could be foreseen.

【 授权许可】

Unknown