Oral microbiota transplantation fights against head and neck radiotherapy-induced oral mucositis in mice

Computational and Structural Biotechnology Journal

Yao Fan¹  Xingzhong Liu²  Saijun Fan²  Huiwen Xiao²  Ming Cui³  Yuan Li³  Bin Wang⁴  Jian Guan⁴  Jia Liu⁴  Jiali Dong⁴  Shuqin Zhang⁴ 

[1] Tianjin Key Laboratory of Radiation Medicine and Molecular Nuclear Medicine, Institute of Radiation Medicine, Chinese Academy of Medical Sciences and Peking Union Medical College, 238 Baidi Road, Tianjin 300192, China;Department of Microbiology, College of Life Sciences, Nankai University, 94 Weijin Road, Tianjin 300071, China;Department of Radiation Oncology, Nanfang Hospital, Southern Medical University, Guangzhou, Guangdong, China;Tianjin Key Laboratory of Radiation Medicine and Molecular Nuclear Medicine, Institute of Radiation Medicine, Chinese Academy of Medical Sciences and Peking Union Medical College, 238 Baidi Road, Tianjin 300192, China;

关键词: Nasal;    Oral and laryngeal cancer;    Radiotherapy;    Radiation-induced oral mucositis;    Oral and gut microbiota;    Lactobacillaceae;   

DOI  :  

来源: DOAJ

【 摘 要 】

Oral mucositis is a common radiotherapy-induced complication among nasal, oral and laryngeal cancer (NOALC) patients. This complication leads to decreased quality of life and has few treatments. Here, fractionated radiation was performed to mimic radiotherapy for NOALCs in mouse models. Oral microbiota transplantation (OMT) mitigated oral mucositis, as judged by reconstructed epithelium and tongue papillae, fewer infiltrated leukocytes and more proliferative cells in the oral epithelium. The gut microbiota impacted oral mucositis progression, and OMT restructured oral and gut bacteria configurations and reprogrammed the gene expression profile of tongue tissues. In vivo silencing of glossal S100 calcium binding protein A9 debilitated the radioprotection of OMT. In light of clinical samples, we identified that patients with different alteration trends of Lactobacillaceae frequency presented different primary lesions and prognoses of NOALC following radiotherapy. Together, our findings provide new insights into the oral-gut microbiota axis and underpin the suggestion that OMT might be harnessed as a novel remedy to fight against oral mucositis in NOALC patients following radiotherapy in preclinical settings. Of note, oral microorganisms, such as Lactobacillaceae, might be employed as biomarkers to predict the prognosis of NOALC with radiotherapy.

【 授权许可】

Unknown