Natural Antioxidant Evaluation: A Review of Detection Methods

Molecules

Danielle Bogo¹  Elaine Silva de Pádua Melo¹  Karine de Cássia Freitas¹  Valter Aragão do Nascimento¹  Gabriela Marcelino¹  Rita de Cássia Avellaneda Guimarães¹  Priscila Aiko Hiane¹  Jenifer da Silva Mendonça¹  Verônica Assalin Zorgetto-Pinheiro¹  Carolina Di Pietro Fernandes²  Marcelo Luiz Brandão Vilela³ 

[1] Graduate Program in Health and Development in the Central-West Region of Brazil, Federal University of Mato Grosso do Sul, Campo Grande 79070-900, Brazil;Group of Spectroscopy and Bioinformatics Applied Biodiversity and Health (GEBABS), Federal University of Mato Grosso do Sul, Campo Grande 79070-900, Brazil;School of Medicine, Federal University of Mato Grosso do Sul, Campo Grande 79070-900, Brazil;

关键词: antioxidant;    bioactive compounds;    secondary metabolites;    antioxidant assay;    in vitro models;    in vivo models;   

DOI  :  10.3390/molecules27113563

来源: DOAJ

【 摘 要 】

Antioxidants have drawn the attention of the scientific community due to being related to the prevention of various degenerative diseases. The antioxidant capacity has been extensively studied in vitro, and different methods have been used to assess its activity. However, the main issues related to studying natural antioxidants are evaluating whether these antioxidants demonstrate a key role in the biological system and assessing their bioavailability in the organism. The majority of outcomes in the literature are controversial due to a lack of method standardization and their proper application. Therefore, this study aims to compile the main issues concerning the natural antioxidant field of study, comparing the most common in vitro methods to evaluate the antioxidant activity of natural compounds, demonstrating the antioxidant activity in biological systems and the role of the main antioxidant enzymes of redox cellular signaling and explaining how the bioavailability of bioactive compounds is evaluated in animal models and human clinical trials.

【 授权许可】

Unknown