The role of scaffold reshaping and disassembly in dynamin driven membrane fission

eLife

Zachary A McDargh¹  Martina Pannuzzo¹  Markus Deserno¹ 

[1] Department of Physics, Carnegie Mellon University, Pittsburgh, United States;

关键词: dynamin;    membrane;    fission;    hemifission;    simulation;   

DOI  :  10.7554/eLife.39441

来源: DOAJ

【 摘 要 】

The large GTPase dynamin catalyzes membrane fission in eukaryotic cells, but despite three decades of experimental work, competing and partially conflicting models persist regarding some of its most basic actions. Here we investigate the mechanical and functional consequences of dynamin scaffold shape changes and disassembly with the help of a geometrically and elastically realistic simulation model of helical dynamin-membrane complexes. Beyond changes of radius and pitch, we emphasize the crucial role of a third functional motion: an effective rotation of the filament around its longitudinal axis, which reflects alternate tilting of dynamin’s PH binding domains and creates a membrane torque. We also show that helix elongation impedes fission, hemifission is reached via a small transient pore, and coat disassembly assists fission. Our results have several testable structural consequences and help to reconcile mutual conflicting aspects between the two main present models of dynamin fission—the two-stage and the constrictase model.

【 授权许可】

Unknown