Chronic Toxicity Study of Neosaxitoxin in Rats

Marine Drugs

Manila Candiracci¹  Nicolas Lobos²  Sebastian Lux²  Ramiro J. Zepeda²  Hugo F. Miranda² 

[1] Anesthesia Department, Brigham and Woman's Hospital, Harvard University, 75 Francis Street, Boston, MA 02115, USA;Molecular and Clinical Pharmacology Program, Institute of Biomedical Sciences, Medical School, University of Chile, Independencia 1027, 8380453 Santiago, Chile;

关键词: neosaxitoxin;    marine toxin;    chronic toxicity;    acute toxicity;   

DOI  :  10.3390/md12095055

来源: DOAJ

【 摘 要 】

Neosaxitoxin (NeoSTX) is a specific reversible blocker of voltage gated sodium channels on excitable cells. In the last decade, it has been tested in a number of interesting clinical trials, however there is still little information available on mammalian toxicity. Rats were treated for 12 weeks with doses of 1, 3 or 6 μg/kg of subcutaneous NeoSTX.At weeks 12 and 17, animals were sacrificed and blood samples collected for hematological and biochemical analysis. Organs were harvested for weight determination and histopathological assessments. The lowest acute toxicity via the intraperitoneal (ip) route was (30.35 μg/kg) and there was no significant difference between intramuscular and subcutaneous routes (11.4 and 12.41 μg/kg). The NeoSTX adiministration did not produce lethality at week 12 and after five weeks of suspension. NeoSTX 6 μg/kg ip produced reductions (p < 0.05) in body weight and food intake, and increased blood level of total and direct bilirubin, GGT and SGOT at week 12; all of these were reversed in the recovery period. NeoSTX 1 and 3 μg/kg ip did not show significant changes with the control group. Histopathological presentations were normal in all groups. This study revealed that NeoSTX is safe in vivo, giving a reliable security margin for its use like a local anesthetic.

【 授权许可】

Unknown