Exposure to naphthalene and β-pinene-derived secondary organic aerosol induced divergent changes in transcript levels of BEAS-2B cells

Environment International

Svenja Offer¹  Christoph Ogris²  Eric Schneider³  Evelyn Kuhn³  Seongho Jeong³  Sebastiano Di Bucchianico³  Stefanie Bauer³  Jan Bendl³  Erwin Karg³  Thorsten Hohaus³  Yue Hu⁴  Fabian Theis⁵  Michal Pardo⁶  Tali Shalit⁶  Andreas Paul⁷  Markus Kalberer⁸  Thomas Adam⁹  Olli Sippula⁹  Martin Sklorz⁹  Zhi-Hui Zhang⁹  Thomas Gröger^1,10  Gert Jakobi^1,11  Astrid Kiendler-Scharr^1,11  Angela Buchholz^1,12  Xin Cao^1,12  Uwe Käfer^1,12  Jürgen Orasche^1,12  Jana Pantzke^1,12  Ramona Brejcha^1,12  Sebastian Oeder^1,12  Anja Huber^1,12  Stephanie Binder^1,12  Christoph Bisig^1,12  Ralf Zimmermann^1,12  Arunas Mesceriakovas^1,12  Annalisa Marsico^1,12  Daniela Gat^1,12  Hendryk Czech^1,12  Narges Rastak^1,12  Christopher P. Rüger^1,12  Elias J. Zimmermann^1,12  Yinon Rudich^1,12  Elena Hartner^1,12  Tamara Kanashova^1,12  Till Ziehm^1,13  Jürgen Schnelle-Kreis^1,14 

[1] Corresponding author.;Institute for Environmental Studies, Faculty of Science, Charles University, Albertov 6, CZE-12800 Prague, Czech Republic;Joint Mass Spectrometry Center (JMSC) at Analytical Chemistry, Institute of Chemistry, University of Rostock, Dr.-Lorenz-Weg 2, D-18059 Rostock, Germany;University of the Bundeswehr Munich, Institute for Chemistry and Environmental Engineering, Werner- Heisenberg-Weg 39, D-85577 Neubiberg, Germany;Department of Applied Physics, University of Eastern Finland, Yliopistonranta 1, P.O. Box 1627, FI-70210 Kuopio, Finland;Department of Earth and Planetary Sciences, Faculty of Chemistry, Weizmann Institute of Science, 234 Herzl Street, POB 26, ISR-7610001 Rehovot, Israel;Department of Environmental Sciences, University of Basel, Klingelbergstr. 27, CH-4056 Basel, Switzerland;Department of Environmental and Biological Sciences, University of Eastern Finland, Yliopistonranta 1, P.O. Box 1627, FI-70210 Kuopio, Finland;Institute of Computational Biology, Helmholtz Zentrum München, Ingolstädter Landstr. 1, D-85764 Neuherberg, Germany;Institute of Energy and Climate Research, Troposphere (IEK-8), Forschungszentrum Jülich GmbH, Wilhelm-Johen-Str., D-52428 Jülich, Germany;Joint Mass Spectrometry Center (JMSC) at Analytical Chemistry, Institute of Chemistry, University of Rostock, Dr.-Lorenz-Weg 2, D-18059 Rostock, Germany;Joint Mass Spectrometry Center (JMSC) at Comprehensive Molecular Analytics (CMA), Helmholtz Zentrum München, Ingolstädter Landstr. 1, D-85764 Neuherberg, Germany;Max-Delbrück-Centrum für Molekulare Medizin (MDC), Robert-Rössle-Str. 10, D-13125 Berlin, Germany;The Mantoux Bioinformatics Institute of the Nancy and Stephen Grand Israel National Center for Personalized Medicine, Weizmann Institute of Science, Rehovot 76100, Israel;

关键词: RNA sequencing;    Soot particles;    Health effects;    Cytotoxicity;    Signaling pathway;    Particulate matter;   

DOI  :  

来源: DOAJ

【 摘 要 】

The health effects of exposure to secondary organic aerosols (SOAs) are still limited. Here, we investigated and compared the toxicities of soot particles (SP) coated with β-pinene SOA (SOAβPin-SP) and SP coated with naphthalene SOA (SOANap-SP) in a human bronchial epithelial cell line (BEAS-2B) residing at the air–liquid interface. SOAβPin-SP mostly contained oxygenated aliphatic compounds from β-pinene photooxidation, whereas SOANap-SP contained a significant fraction of oxygenated aromatic products under similar conditions. Following exposure, genome-wide transcriptome responses showed an Nrf2 oxidative stress response, particularly for SOANap-SP. Other signaling pathways, such as redox signaling, inflammatory signaling, and the involvement of matrix metalloproteinase, were identified to have a stronger impact following exposure to SOANap-SP. SOANap-SP also induced a stronger genotoxicity response than that of SOAβPin-SP. This study elucidated the mechanisms that govern SOA toxicity and showed that, compared to SOAs derived from a typical biogenic precursor, SOAs from a typical anthropogenic precursor have higher toxicological potency, which was accompanied with the activation of varied cellular mechanisms, such as aryl hydrocarbon receptor. This can be attributed to the difference in chemical composition; specifically, the aromatic compounds in the naphthalene-derived SOA had higher cytotoxic potential than that of the β-pinene-derived SOA.

【 授权许可】

Unknown