Influence of Polyelectrolyte Multilayer Properties on Bacterial Adhesion Capacity

Polymers

Jasmina Salopek¹  Davor Kovačević¹  Rok Pratnekar²  Karmen Godič Torkar²  Klemen Bohinc²  Anže Abram³  Goran Dražić³ 

[1] Department of Chemistry, Faculty of Science, University of Zagreb, Zagreb 10000, Croatia;Faculty of Health Sciences, Ljubljana 1000, Slovenia;Jožef Stefan Institute, Ljubljana 1000, Slovenia;

关键词: bacterial adhesion;    Pseudomonas aeruginosa;    polyelectrolyte multilayers;    poly(allylamine hydrochloride);    poly(4-styrenesulfonate);    scanning electron microscopy;    zeta potential;   

DOI  :  10.3390/polym8100345

来源: DOAJ

【 摘 要 】

Bacterial adhesion can be controlled by different material surface properties, such as surface charge, on which we concentrate in our study. We use a silica surface on which poly(allylamine hydrochloride)/sodium poly(4-styrenesulfonate) (PAH/PSS) polyelectrolyte multilayers were formed. The corresponding surface roughness and hydrophobicity were determined by atomic force microscopy and tensiometry. The surface charge was examined by the zeta potential measurements of silica particles covered with polyelectrolyte multilayers, whereby ionic strength and polyelectrolyte concentrations significantly influenced the build-up process. For adhesion experiments, we used the bacterium Pseudomonas aeruginosa. The extent of adhered bacteria on the surface was determined by scanning electron microscopy. The results showed that the extent of adhered bacteria mostly depends on the type of terminating polyelectrolyte layer, since relatively low differences in surface roughness and hydrophobicity were obtained. In the case of polyelectrolyte multilayers terminating with a positively charged layer, bacterial adhesion was more pronounced than in the case when the polyelectrolyte layer was negatively charged.

【 授权许可】

Unknown