Convergent antibody responses to the SARS-CoV-2 spike protein in convalescent and vaccinated individuals

Cell Reports

Rachel E. Sutton¹  Carly R. Cabel²  Luke Myers²  Rita E. Chen³  Elad Binshtein⁴  James E. Crowe, Jr.⁵  Curtis A. Thorne⁵  Emma S. Winkler⁶  Samuel Day⁶  Elaine C. Chen⁷  Robert H. Carnahan⁸  Samuel K. Campos⁸  Michael S. Diamond⁸  Andrew Trivette⁹  Jazmean K. Williams⁹  Pavlo Gilchuk⁹  Seth J. Zost⁹  Jessica Rodriguez⁹  Shuaizhi Li⁹  Naveenchandra Suryadevara⁹  Benjamin J. Doranz⁹  Edgar Davidson⁹ 

[1] Cancer Biology Graduate Interdisciplinary Program, University of Arizona, Tucson, AZ 85724, USA;Department of Medicine, Washington University School of Medicine, Saint Louis, MO 63110, USA;Molecular Medicine, University of Arizona College of Medicine, Tucson, AZ 85724, USA;;Department of Cellular &Department of Immunobiology, University of Arizona College of Medicine, Tucson, AZ 85724, USA;Department of Pathology and Immunology, Washington University School of Medicine, Saint Louis, MO 63110, USA;Department of Pathology, Microbiology, and Immunology, Vanderbilt University Medical Center, Nashville, TN 37232, USA;Integral Molecular, Inc., Philadelphia, PA 19104, USA;Vanderbilt Vaccine Center, Vanderbilt University Medical Center, Nashville, TN 37232, USA;

关键词: coronavirus;    SARS-CoV-2;    SARS-CoV;    COVID-19;    antibodies;    monoclonal;   

DOI  :  

来源: DOAJ

【 摘 要 】

Summary: Unrelated individuals can produce genetically similar clones of antibodies, known as public clonotypes, which have been seen in responses to different infectious diseases, as well as healthy individuals. Here we identify 37 public clonotypes in memory B cells from convalescent survivors of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) infection or in plasmablasts from an individual after vaccination with mRNA-encoded spike protein. We identify 29 public clonotypes, including clones recognizing the receptor-binding domain (RBD) in the spike protein S1 subunit (including a neutralizing, angiotensin-converting enzyme 2 [ACE2]-blocking clone that protects in vivo) and others recognizing non-RBD epitopes that bind the S2 domain. Germline-revertant forms of some public clonotypes bind efficiently to spike protein, suggesting these common germline-encoded antibodies are preconfigured for avid recognition. Identification of large numbers of public clonotypes provides insight into the molecular basis of efficacy of SARS-CoV-2 vaccines and sheds light on the immune pressures driving the selection of common viral escape mutants.

【 授权许可】

Unknown