Immunomodulatory and Prebiotic Effects of 2′-Fucosyllactose in Suckling Rats

Frontiers in Immunology

Bernd Stahl¹  Sebastian Tims¹  Karen Knipping²  Johan Garssen²  Jordi Mayneris-Perxachs³  Belinda van't Land⁴  Malén Massot-Cladera⁵  Ignasi Azagra-Boronat⁵  Margarida Castell⁵  M. José Rodríguez-Lagunas⁵  Francisco J. Pérez-Cano⁵  Àngels Franch⁵ 

[1] Danone Nutricia Research, Utrecht, Netherlands;Division of Pharmacology, Faculty of Science, Utrecht Institute for Pharmaceutical Sciences, Utrecht University, Utrecht, Netherlands;Eurecat, Centre Tecnològic de Catalunya, Technological Unit of Nutrition and Health and Technological Unit of Omic Sciences, Reus, Spain;Laboratory of Translational Immunology, University Medical Centre Utrecht, Wilhelmina Children's Hospital, Utrecht, Netherlands;Nutrition and Food Safety Research Institute (INSA-UB), Santa Coloma de Gramenet, Spain;Physiology Section, Department of Biochemistry and Physiology, Faculty of Pharmacy and Food Science, University of Barcelona, Barcelona, Spain;

关键词: 2′-FL;    microbiota;    HMO;    immunoglobulins;    metabolomics;   

DOI  :  10.3389/fimmu.2019.01773

来源: DOAJ

【 摘 要 】

Human milk oligosaccharides are unconjugated complex glycans present in high concentration in human milk that serve as pre-biotics and immunomodulators. They are not primarily absorbed or metabolized by the infant and reach the lower part of the intestinal tract unaltered. One of the main oligosaccharides found in human milk is 2′-fucosyllactose (2′-FL). This study aimed to investigate the effects of daily oral administration of 2′-FL in healthy suckling rats. From days 2 to 16 of life, rats were daily given the oligosaccharide (2′-FL) or vehicle (REF), weighed and their stool characteristics were assessed. On days 8 and 16 of life the morphometry, intestinal architecture, and cytokine release, mesenteric lymph nodes cell composition, plasma immunoglobulin concentrations, fecal microbiota composition, cecal short-chain fatty acids content, and the urinary metabolic profile were assessed. Animals given 2′-FL showed higher plasma IgG and IgA and more T cell subsets in the mesenteric lymph nodes on day 16. Moreover, at intestinal level, villus heights, and areas were increased on day 8. Cecal samples displayed a higher Lactobacillus proportion and a different urinary metabolic profile was observed on day 8, and a higher proportion of butyrate on day 16. In conclusion, supplementation of 2′-FL in early life has a pre-biotic and intestinal trophic effect and promotes maturation of the immune system.

【 授权许可】

Unknown