Layered Double Hydroxide Nanomaterials Encapsulating Angelica gigas Nakai Extract for Potential Anticancer Nanomedicine

Frontiers in Pharmacology

Jae-Min Oh¹  Ae-Jin Choi¹  Tae-il Kim³  Gyeong Jin Lee³  Tae-Hyun Kim⁴  Hyoung-Jun Kim⁴ 

[1] Biomaterials Science and Engineering, College of Agriculture and Life Sciences, Seoul National University, Seoul, South Korea;Department of Agrofood Resources, National Institute of Agricultural Sciences of RDA, Wanju, South Korea;;Department of Biosystems &Department of Chemistry and Medical Chemistry, College of Science and Technology, Yonsei University, Wonju, South Korea;Department of Physics, Chemistry and Pharmacy, University of Southern Denmark, Odense, Denmark;

关键词: layered double hydroxide;    Angelica gigas Nakai;    anticancer activity;    decursin species;    nanobiohybrid;    phytochemical;   

DOI  :  10.3389/fphar.2018.00723

来源: DOAJ

【 摘 要 】

We prepared hybrids consisting of Angelica gigas Nakai (AGN) root or flower extract and layered double hydroxide (LDH) for potential anticancer nanomedicine, as decursin species (DS) in AGN are known to have anticancer activity. Dimethylsulfoxide solvent was determined hybridization reaction media, as it has affinity to both AGN and LDH moiety. In order to develop inter-particle spaces in LDH, a reversible dehydration-rehydration, so-called reconstruction route, was applied in AGN-LDH hybridization. Quantitative analyses on AGN-LDH hybrids indicated that the content of DS was two times more concentrated in the hybrids than in extract itself. Using X-ray diffraction, FT-IR spectroscopy, scanning electron microscopy, and zeta-potential measurement, we found that AGN extract moiety was incorporated into inter-particle spaces of LDH nanoparticles during the reconstruction reaction. Time-dependent DS release from hybrids at pH 7.4 (physiological condition) and pH 4.5 (lysosomal condition) exhibited a pH-dependent release of extract-incorporated LDH hybrids. An anticancer activity test using HeLa, A549, and HEK293T cells showed that the AGN-LDH hybrid, regardless of extract type, showed enhanced anticancer activity compared with extract alone at an equivalent amount of DS, suggesting a nanomedicine effect of AGN-LDH hybrids.

【 授权许可】

Unknown