Intradermal-delivered DNA vaccine induces durable immunity mediating a reduction in viral load in a rhesus macaque SARS-CoV-2 challenge model

Cell Reports Medicine

Kate E. Broderick¹  Laurent Pessaint²  Anthony Cook²  Brad Finneyfrock²  Mark G. Lewis²  Hanne Andersen²  John Harrison²  Alan Dodson²  Katherine Schultheis³  Arthur Doan³  Laurent M. Humeau³  Jewell N. Walters³  Dustin Elwood³  Viviane M. Andrade³  Zeena Eblimit³  Diana Guimet³  Alison Generotti³  Igor Maricic³  J. Joseph Kim³  Trevor R.F. Smith³  Stephanie J. Ramos³  Pratik Bhojnagarwala⁴  Daniel W. Kulp⁴  Sophia M. Reeder⁴  Ebony N. Gary⁴  Jihae Choi⁴  Elizabeth Parzych⁴  Susanne N. Walker⁴  Neethu Chokkalingam⁴  Faraz I. Zaidi⁴  Ami Patel⁴  Opeyemi S. Adeniji⁴  Emma L. Reuschel⁴  Mansi Purwar⁴  Kar Muthumani⁴  David B. Weiner⁴  Yaya Dia⁴  Ziyang Xu⁴  Kevin Y. Kim⁴  Edgar Tello-Ruiz⁴  Mohamed Abdel-Mohsen⁴  Nicholas J. Tursi⁴ 

[1] Corresponding author;Bioqual, Rockville, MD 20852, USA;Inovio Pharmaceuticals, Plymouth Meeting, PA 19462, USA;Vaccine and Immunotherapy Center, The Wistar Institute, Philadelphia, PA 19104, USA;

关键词: SARS-CoV-2;    COVID-19;    coronavirus;    ID DNA vaccine;    electroporation;    macaque;   

DOI  :  

来源: DOAJ

【 摘 要 】

Summary: Coronavirus disease 2019 (COVID-19), caused by the SARS-CoV-2 virus, has had a dramatic global impact on public health and social and economic infrastructures. Here, we assess the immunogenicity and anamnestic protective efficacy in rhesus macaques of an intradermal (i.d.)-delivered SARS-CoV-2 spike DNA vaccine, INO-4800, currently being evaluated in clinical trials. Vaccination with INO-4800 induced T cell responses and induced spike antigen and RBD binding antibodies with ADCP and ADCD activity. Sera from the animals neutralized both the D614 and G614 SARS-CoV-2 pseudotype viruses. Several months after vaccination, animals were challenged with SARS-CoV-2 resulting in rapid recall of anti-SARS-CoV-2 spike protein T cell and neutralizing antibody responses. These responses were associated with lower viral loads in the lung. These studies support the immune impact of INO-4800 for inducing both humoral and cellular arms of the adaptive immune system, which are likely important for providing durable protection against COVID-19 disease.

【 授权许可】

Unknown