期刊论文详细信息
Microbiology Spectrum
Genomic and Chemical Decryption of the Bacteroidetes Phylum for Its Potential to Biosynthesize Natural Products
Peter E. Hammann1  Christoph Hartwig2  Jens Glaeser2  Yolanda Kleiner2  Maria A. Patras2  Andreas Vilcinskas2  Michael Marner2  Till F. Schäberle2  André Billion2  Marius Spohn2  Stephan Brinkmann2  Benedikt Leis2  Michael Kurz3  Luigi Toti3  Christoph Pöverlein3  Armin Bauer3 
[1]Evotec International GmbH, Göttingen, Germany
[2]Fraunhofer Institute for Molecular Biology and Applied Ecology (IME), Branch for Bioresources, Giessen, Germany
[3]Sanofi-Aventis Deutschland GmbH, Frankfurt am Main, Germany
关键词: natural products;    antifungal;    NRPS;    metabolomics;    genomics;    Bacteroidetes;   
DOI  :  10.1128/spectrum.02479-21
来源: DOAJ
【 摘 要 】
ABSTRACT With progress in genome sequencing and data sharing, 1,000s of bacterial genomes are publicly available. Genome mining—using bioinformatics tools in terms of biosynthetic gene cluster (BGC) identification, analysis, and rating—has become a key technology to explore the capabilities for natural product (NP) biosynthesis. Comprehensively, analyzing the genetic potential of the phylum Bacteroidetes revealed Chitinophaga as the most talented genus in terms of BGC abundance and diversity. Guided by the computational predictions, we conducted a metabolomics and bioactivity driven NP discovery program on 25 Chitinophaga strains. High numbers of strain-specific metabolite buckets confirmed the upfront predicted biosynthetic potential and revealed a tremendous uncharted chemical space. Mining this data set, we isolated the new iron chelating nonribosomally synthesized cyclic tetradeca- and pentadecalipodepsipeptide antibiotics chitinopeptins with activity against Candida, produced by C. eiseniae DSM 22224 and C. flava KCTC 62435, respectively. IMPORTANCE The development of pipelines for anti-infectives to be applied in plant, animal, and human health management are dried up. However, the resistance development against compounds in use calls for new lead structures. To fill this gap and to enhance the probability of success for the discovery of new bioactive natural products, microbial taxa currently underinvestigated must be mined. This study investigates the potential within the bacterial phylum Bacteroidetes. A combination of omics-technologies revealed taxonomical hot spots for specialized metabolites. Genome- and metabolome-based analyses showed that the phylum covers a new chemical space compared with classic natural product producers. Members of the Bacteroidetes may thus present a promising bioresource for future screening and isolation campaigns.
【 授权许可】

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