Hplc Analysis of Certain Phenolic Compounds and Carotenoid in Two Calendula Species

Turkish Journal of Agriculture: Food Science and Technology

Cüneyt Akı¹  Nergis Kaya² 

[1] Department of Biology, Faculty of Arts and Sciences, Molecular Biology USA, Terzioğlu Campus, Çanakkale Eighteen Mart University 17020 Çanakkale;Department of Food Processing, Food Technology Program, Biga Vocational School, Eighteen Mart University of Çanakkale, 17200 Çanakkale;

关键词: calendula;    hplc;    fenolik bileşikler;    beta karoten;    kaemferol;   

DOI  :  10.24925/turjaf.v8i12.2764-2769.4098

来源: DOAJ

【 摘 要 】

Calendula officinalis (pot marigold), a medicinal ornamental plant belonging to the Asteraceae family, has various medicinal activities such as anticancer, antimicrobial, antioxidant and antitumor. The retention time of quercetin (6.445 min), kaempferol (11.246 min), caffeic acid (2.333 min), beta carotene (9.614 min) standards, which were planned to be analyzed by HPLC, was determined. The HPLC conditions that are most suitable for these metabolites have been determined. The amounts of quercetin, kaempferol, caffeic acid and beta carotene in the seedling leaves of these two Calendula species on the 0., 15., 30., 45., 60. days were compared. Caffeic acid and beta carotene have been detected in the leaves. However, quercetin and kaempferol compounds were not detected. It was determined that the caffeic acid and beta carotene amounts of C. officinalis species were higher than that of C. arvensis. The highest caffeic acid in both C. officinalis and C. arvensis seedlings (0.4042±0.0123 µg/g dry weight for C. officinalis, 0.1918±0.0437 µg / g dry weight for C. arvensis) and beta carotene amounts (0.8520±0.0692 µg/g dry weight for C. officinalis, 0.6389±0.0189 µg/g dry weight for C. arvensis) were determined on 60. day. In addition, it was determined that the amount of these metabolites differed according to the seedling development period.

【 授权许可】

Unknown