Lactobacillus acidophilus JCM 1132 Strain and Its Mutant with Different Bacteriocin-Producing Behaviour Have Various In Situ Effects on the Gut Microbiota of Healthy Mice

Microorganisms

Jianxin Zhao¹  Enriqueta Garcia-Gutierrez²  Zhenmin Liu³  Hao Zhang⁴  Gang Wang⁴  Yunxia Yu⁴  Linlin Wang⁴  Wei Chen⁴  Peijun Tian⁴  Xing Jin⁴  Yufeng He⁴ 

[1]Food Co., Ltd., Shanghai 200436, China

[2]Gut Microbes and Health Institute Strategic Programme, Quadram Institute Bioscience, Norwich NR4 7UQ, UK

[3]

[4]State Key Laboratory of Dairy Biotechnology, Shanghai Engineering Research Center of Dairy Biotechnology, Dairy Research Institute, Bright Dairy &State Key Laboratory of Food Science and Technology, Jiangnan University, Wuxi 214122, China

关键词: lactobacillus acidophilus;    bacteriocin;    short-chain fatty acids;    gut microbiota;    in situ effects;   

DOI  :  10.3390/microorganisms8010049

来源: DOAJ

【 摘 要 】

The production of bacteriocin is considered to be a probiotic trait of lactic acid bacteria (LAB). However, not all strains of LAB harbour bacteriocin genes, even within the same species. Moreover, the effects of bacteriocins on the host gut microbiota and on host physiological indicators are rarely studied. This study evaluated the effects of the bacteriocin-producing Lactobacillus acidophilus strain JCM1132 and its non-producing spontaneous mutant, L. acidophilus CCFM720, on the physiological statuses and gut microbiota of healthy mice. Mice that received the bacteriocin-producing strain JCM1132 exhibited reduced water and food intake. Furthermore, the administration of these strains induced significant changes in the compositional abundance of faecal microbiota at the phylum and genus levels, and some of these changes were more pronounced after one week of withdrawal. The effects of CCFM720 treatment on the gut microbiota seemed to favour the prevention of metabolic diseases to some extent. However, individuals that received JCM1132 treatment exhibited weaker inflammatory responses than those that received CCFM720 treatment. Our results indicate that treatment with bacteriocin-producing or non-producing strains can have different effects on the host. Accordingly, this trait should be considered in the applications of LAB.

【 授权许可】

Unknown