Heterologous Expression of Secondary Metabolite Genes in Trichoderma reesei for Waste Valorization

Journal of Fungi

Maria Ambilika¹  Elizabeth Skellam¹  Mary L. Shenouda¹  Russell J. Cox¹ 

[1] Institute for Organic Chemistry and Biomolekulares Wirkstoffzentrum (BMWZ), Schneiderberg 38, 30167 Hannover, Germany;

关键词: heterologous expression;    PKS-NRPS;    PKS;    waste valorization;    microbial cell factory;    Trichoderma reesei;   

DOI  :  10.3390/jof8040355

来源: DOAJ

【 摘 要 】

Trichoderma reesei (Hypocrea jecorina) was developed as a microbial cell factory for the heterologous expression of fungal secondary metabolites. This was achieved by inactivation of sorbicillinoid biosynthesis and construction of vectors for the rapid cloning and expression of heterologous fungal biosynthetic genes. Two types of megasynth(et)ases were used to test the strain and vectors, namely a non-reducing polyketide synthase (nr-PKS, aspks1) from Acremonium strictum and a hybrid highly-reducing PKS non-ribosomal peptide synthetase (hr-PKS-NRPS, tenS + tenC) from Beauveria bassiana. The resulting engineered T. reesei strains were able to produce the expected natural products 3-methylorcinaldehyde and pretenellin A on waste materials including potato, orange, banana and kiwi peels and barley straw. Developing T. reesei as a heterologous host for secondary metabolite production represents a new method for waste valorization by the direct conversion of waste biomass into secondary metabolites.

【 授权许可】

Unknown