Gut Pathogens | |
Comparative genomic analysis reveals genetic features related to the virulence of Bacillus cereus FORC_013 | |
Hyun-Jin Koo1  Heebal Kim1  Ju-Hoon Lee2  Han Young Chung2  Sang Ho Choi2  Sangryeol Ryu2  Suyeon Kim2  Kwondo Kim3  Sojin Ahn3  | |
[1] Department of Agricultural Biotechnology and Research Institute of Agriculture and Life Sciences, Seoul National University;Food-Borne Pathogen Omics Research Center (FORC), Seoul National University;Interdisciplinary Program in Bioinformatics, Seoul National University; | |
关键词: Bacillus cereus; Virulence; Comparative genomics; Positive selection; Unique genes; | |
DOI : 10.1186/s13099-017-0175-z | |
来源: DOAJ |
【 摘 要 】
Abstract Background Bacillus cereus is well known as a gastrointestinal pathogen that causes food-borne illness. In the present study, we sequenced the complete genome of B. cereus FORC_013 isolated from fried eel in South Korea. To extend our understanding of the genomic characteristics of FORC_013, we conducted a comparative analysis with the published genomes of other B. cereus strains. Results We fully assembled the single circular chromosome (5,418,913 bp) and one plasmid (259,749 bp); 5511 open reading frames (ORFs) and 283 ORFs were predicted for the chromosome and plasmid, respectively. Moreover, we detected that the enterotoxin (NHE, HBL, CytK) induces food-borne illness with diarrheal symptom, and that the pleiotropic regulator, along with other virulence factors, plays a role in surviving and biofilm formation. Through comparative analysis using the complete genome sequence of B. cereus FORC_013, we identified both positively selected genes related to virulence regulation and 224 strain-specific genes of FORC_013. Conclusions Through genome analysis of B. cereus FORC_013, we identified multiple virulence factors that may contribute to pathogenicity. These results will provide insight into further studies regarding B. cereus pathogenesis mechanism at the genomic level.
【 授权许可】
Unknown