Keystone Taxa Lactiplantibacillus and Lacticaseibacillus Directly Improve the Ensiling Performance and Microflora Profile in Co-Ensiling Cabbage Byproduct and Rice Straw

Microorganisms

Guilong Zhang¹  Jingxian Zhang²  Guilin Du²  Zhiguo Ma²  Xiangcen Liu²  Jiping Shi²  Xiang Li²  Chenyang Yuan²  Baoguo Zhang² 

[1] Agro-Environmental Protection Institute, Ministry of Agriculture and Rural Affairs, Tianjin 300191, China;Lab of Biorefinery, Shanghai Advanced Research Institute, Chinese Academy of Sciences, No. 99 Haike Road, Pudong, Shanghai 201210, China;

关键词: vegetable waste;    silage;    Lactiplantibacillus plantarum inoculation;    microbial community dynamics;    microbial phenotypes;    potential pathogen;   

DOI  :  10.3390/microorganisms9051099

来源: DOAJ

【 摘 要 】

Ensiling has been widely applied to cope with agricultural solid waste to achieve organic waste valorization and relieve environmental pressure and feedstuff shortage. In this study, co-ensiling of cabbage leaf byproduct and rice straw was performed with inoculation of Lactiplantibacillusplantarum (LP) to investigate the effects of inoculation on ensiling performance and microflora profiles. Compared to the control, LP inoculation preserved more dry matter (DM) content (283.4 versus 270.9 g·kg⁻¹ fresh matter (FM) on day 30), increased lactic acid (LA) content (52.1 versus 35.8 g·kg⁻¹ dry matter on day 15), decreased pH (3.55 versus 3.79 on day 15), and caused accumulation of acetic acid (AA), butyric acid (BA), and ammonia. The investigation showed that LP inoculation modified microflora composition, especially resisting potential pathogens and enriching more lactic acid bacteria (LAB) (p < 0.05). Moreover, Lactiplantibacillus and Lacticaseibacillus were identified as the keystone taxa that influenced physicochemical properties and interactions in microflora. They were also the main functional species that directly restrained undesirable microorganisms (p < 0.05), rather than indirectly working via metabolite inhibition and substrate competition (p > 0.05). The results of this present study improve the understanding of the underlying effect of LP inoculation on improving silage quality and facilitate the bio-transformation of cabbage byproduct and rice straw as animal feed.

【 授权许可】

Unknown