Tissue-Specific Metabolic Reprogramming during Wound-Induced Organ Formation in Tomato Hypocotyl Explants

International Journal of Molecular Sciences

Cristina Martínez-Andújar¹  Alfonso Albacete¹  José Manuel Pérez-Pérez²  Ana Belén Sánchez-García²  Eduardo Larriba² 

[1] Department of Plant Nutrition, Centro de Edafología y Biología Aplicada del Segura (CEBAS-CSIC), Campus Universitario de Espinardo, Espinardo, 30100 Murcia, Spain;Instituto de Bioingeniería, Universidad Miguel Hernández, 03202 Elche, Spain;

关键词: de novo shoot apical meristem formation;    de novo root regeneration;    glycolysis/gluconeogenesis;    photorespiration;    photosynthesis;    time-course bulk RNA-Seq;   

DOI  :  10.3390/ijms221810112

来源: DOAJ

【 摘 要 】

Plants have remarkable regenerative capacity, which allows them to survive tissue damage after exposure to biotic and abiotic stresses. Some of the key transcription factors and hormone crosstalk mechanisms involved in wound-induced organ regeneration have been extensively studied in the model plant Arabidopsis thaliana. However, little is known about the role of metabolism in wound-induced organ formation. Here, we performed detailed transcriptome analysis and used a targeted metabolomics approach to study de novo organ formation in tomato hypocotyl explants and found tissue-specific metabolic differences and divergent developmental pathways. Our results indicate that successful regeneration in the apical region of the hypocotyl depends on a specific metabolic switch involving the upregulation of photorespiratory pathway components and the differential regulation of photosynthesis-related gene expression and gluconeogenesis pathway activation. These findings provide a useful resource for further investigation of the molecular mechanisms involved in wound-induced organ formation in crop species such as tomato.

【 授权许可】

Unknown