Direct visualization of floppy two-dimensional DNA origami using cryogenic electron microscopy

iScience

Paul M. Chaikin¹  Nadrian C. Seeman²  Galio Guo³  Do-Nyun Kim⁴  Jae Young Lee⁴  Xiao Fan⁵  Feng Zhou⁶  Heng Ni⁶  Yimo Han⁶  Nan Yao⁷ 

[1] Corresponding author;Department of Chemistry, New York University, New York, NY 10003, USA;Department of Materials Science and NanoEngineering, Rice University, Houston, TX 77005, USA;Department of Mechanical Engineering, Seoul National University, Seoul 08826, Republic of Korea;Department of Molecular Biology, Princeton University, Princeton, NJ 08544, USA;Department of Physics, New York University, New York, NY 10003, USA;PRISM Imaging and Analysis Center, Princeton University, Princeton, NJ 08544, USA;

关键词: Materials science;    Materials chemistry;    Biomaterials;    Materials characterization;   

DOI  :  

来源: DOAJ

【 摘 要 】

Summary: Two-dimensional (2D) DNA origami that is capable of self-assembling into complex 2D and 3D geometries pave the way for a bottom-up synthesis for various applications in nano/biotechnology. Here, we directly visualized the aqueous structure of 2D DNA origami cross-tiles and their assemblies using cryogenic electron microscopy. We uncovered flexible arms in cross-tile monomers and designated inter-tile folding. In addition, we observed the formation of clusters and stacks of DNA cross-tiles in solution, which could potentially affect the interaction and assembly of DNA origami. Finally, we quantitatively evaluated the flexibility of DNA origami in solution using finite element analysis. Our discovery has laid the foundation for investigating the dynamic structures of 2D DNA origami assemblies in solution, providing insights regarding the self-assembly and self-replication mechanisms of 2D DNA origami.

【 授权许可】

Unknown