Expression of Spermine Oxidase Is Associated with Colorectal Carcinogenesis and Prognosis of Patients

Biomedicines

Sooyoun Kim¹  Sanghyun Roh¹  Dongjun Jeong¹  Moon Soo Lee²  Dong Hyun Kang²  Tae Sung Ahn²  Moo-Jun Baek²  Chang-Jin Kim³  Hyoung Jong Kwak³  Inpyo Hong⁴  Hyeongjoo Kim⁴  Doyeon Kim⁴ 

[1] Department of Pathology, College of Medicine, Soonchunhyang University, 31 Soonchunhyang 6 gil, Dongnam-gu, Cheonan 31151, Chungcheongnam-do, Korea;Department of Surgery, College of Medicine, Soonchunhyang University, 31 Soonchunhyang 6 gil, Dongnam-gu, Cheonan 31151, Chungcheongnam-do, Korea;Research Institute of Clinical Medicine, Woori Madi Medical Center, 111 Baekjedae-ro, Wansan-gu, Jeonju 55082, Jeollabuk-do, Korea;Soonchunhyang Medical Science Research Institute, College of Medicine, Soonchunhyang University, 31 soonchunhyang 6 gil, Dongnam-gu, Cheonan 31151, Chungcheongnam-do, Korea;

关键词: colorectal cancer (CRC);    SMOX;    prognostic marker;    therapeutic target;   

DOI  :  10.3390/biomedicines10030626

来源: DOAJ

【 摘 要 】

Uncovering tumor markers of colorectal cancer is important for the early detection and prognosis of the patients. Spermine oxidase (SMOX) is upregulated in various cancers. The present study aims to explore the biologic function and expression patterns of SMOX in colorectal cancer (CRC), the third most common type of cancer worldwide. We used quantitative real-time PCR, Western blot, and in vitro functional studies in four CRC cell lines knocked down by SMOX siRNA and immunohistochemistry in 350 cases of CRC tissues. The results showed that SMOX was overexpressed in CRC cell lines and clinical samples. SMOX overexpression in tumor tissues was an independent prognostic factor, worsening overall survival (p = 0.001). The knock-down of SMOX inhibited CRC cell proliferation, invasion, and soft agar colony formation, uncovering its carcinogenic functions. This study indicated that SMOX overexpression could be an important oncogene in CRC and might serve as a valuable prognostic marker and potential therapeutic target for CRC.

【 授权许可】

Unknown